Cathepsin B staining in Jurkat T cells after treatment with inhibitors. Jurkat cells were treated with 10 μM CA-074Me for 3 hours (blue histogram), 10μM Leupeptin for 1 hour (red histogram) or were untreated (green histogram). They were then stained for 1 hour with R110-(RR)2. After incubation with the R110-(RR)2 substrate, the samples were analyzed by flow cytometry using a ZE5 Cell Analyzer (488 nm excitation and a 525/35 emission filter). Treatment with CA-074Me or Leupeptin decreased the fluorescence signal detected compared to the untreated control (see table), which displayed a baseline level of cathepsin activity
Supplier Page from Bio-Rad for GREEN CATHEPSIN B KIT