Description
This ELISA is designed for the quantitative determination of ß2-microglobulin. In a first inicubation step, ß2-microglobulin is bound to an immobilised antibody. Then a peroxidase-labelled anti-ß2-microglobulin-antibody is added and a sandwich of capture antibody – ß2-microglobulin – peroxidase-conjugate is formed. Tetra-methylbenzidine (TMB) is used as peroxidase substrate. Finally, an acidic stop solu-tion is added to terminate the reaction. The colour changes from blue to yellow. The intensitiy of the colour is directly proportional to the concentration of ß2-microglobulin. A dose response curve of the absorbance unit (optical density, OD at 450 nm) vs. concentration is generated, using the values obtained from the standards. ß2-mi-croglobulin, present in the patient samples, is determined directly from this curve