Description
The blood samples are enzymatically pre-treated and then transferred in the wells of a microtiter plate[PLATE] coated with Lactobacillusfermentum.The addition of vitamin B1 in either standards [STD],control [CTRL1,CTRL2] or samples gives a vitamin B1-dependent growth response until vitamin B1 is consumed.After incubation at 37 °C for 48 hours, the growth of Lactobacillus fermentum is measured turbidimetrically at 610 - 630 nm (alternatively at 540 - 550 nm) in an ELISA-reader and a standard curve is generated from the dilution series.The amount of vitamin B1 is directly proportional to the turbidity