Description
The Salivary Progesterone EIA kit is based on the competition principal and microplate separation. Progesterone calibrators and unknown amounts of progesterone in saliva samples compete with a fixed amount of progesterone conjugated to horse radish peroxidase (Progesterone-HRP) for binding sites with a rabbit progesterone monoclonal antiserum bound to GARGG (goat anti-rabbit gamma globulin) coated wells of a microplate. After incubation, unbound components are washed away, enzyme substrate solution is added and a blue color formed. This reaction is stopped with an acid solution to produce a yellow color. The optical density is then read at 450 nm. The amount of Progesterone-HRP detected is inversely proportional to the amount of progesterone in a sample