Description
Serum samples are diluted and added into the microtiter plate wells coated with Lactobacillus plantarum which metabolizes pantothenic acid.The presence of pantothenic acid both in standards [STD] and samples gives a pantothenic acid-dependent growth response.After incubation at 37°C for 24 hours, the growth of Lactobacillus plantarum is measured turbidimetrically at 610 - 630 nm (alternative at 540 - 550 nm) in an ELISA-reader.A dose response curve of absorbance unit (optical density,OD at 610 nm) vs. concentration is generated using the values obtained from standard.Pantothenic acid present in the patient samples is determined directly from this curve