Description
The principle of the following enzyme immunoassay test follows the typical competitive binding scenario. Competition occurs between an unlabelled antigen (present in standards, controls and patient samples) and an enzyme-labelled antigen (conjugate) for a limited number of antibod binding sites on the microplate. The washing and decanting procedures remove unbound materials. A fter the washing step, the enzyme substrate is added. The enzymatic reaction is terminated by addition of the stopping solution. The absorbance is measured on a microtiter plate reader. The intensity of the colour formed is inversely proportional to the concentration of 17α-OHP in the sample. A s et of standards is used to plot a standard curve from which the amount of 17α-OHP in patient samples and controls can be directly read