Description
The serum is pre-treated and diluted with a buffer mixture,and then sampleswere transferred in the wells of a microtiter plate [PLATE] coated with Lactobacillusdelbrueckii subsp.lactis.The addition of vitamin B12 in either standards [STD] or samples gives a vitamin B12-dependent growth response until vitamin B12 is consumed. After incubation at 37°C for 44 - 48 hours, the growth of Lactobacillusplantarum is measured turbidimetrically at 610 - 630 nm (alternatively at 540 - 550 nm) in an ELISA-reader and a standard curve isgenerated from the dilution series. The amount of vitamin B12 is directly proportional to the turbidity