Description
This ELISA is designed for the quantitative determination of underglycosylated bone sialoprotein in serum and EDTA plasma. The test principle is based on interaction of the antigen in the samples or standards and with the antibody coated on the wells of the microtiter plate. A peroxidase-conjugated secondary antibody is used for detection and quantification, and tetramethylbenzidine (TMB) as a peroxidase substrate. The enzymatic reaction is terminated by an acidic stop solution. A dose response curve of the absorbance unit (optical density, OD at 450 nm) vs. concentration is generated, using the values obtained from the standard. Bone sialoprotein, present in the patient samples, is determined directly from this curve