Fig 1: A, Mean log10 perfusate concentration (pg/mL) of cytokines at 0, 1, and 2 h of NMP. Solid and dashed lines indicate pro- and anti-inflammatory cytokines, respectively. Error bars represent SE. B and C, Radar chart showing the mean log10 concentrations (pg/mL) of 13-plex cytokine measurements in kidney perfusate samples (n = 41) after 2 h NMP (1-h NMP data not shown). Profiles are stratified for (B) posttransplant graft function (IGF [n = 16], DGF [n = 22], and PNF [n = 3]), and (C) BPAR (no BPAR [n = 35], BPAR [n = 4], and borderline [not shown, n = 2]). BPAR, biopsy-proven acute rejection (within the first 2 wk after transplantation); CCL2, C-C motif chemokine ligand 2; DGF, delayed graft function (need for dialysis in the first week after transplantation); IGF, immediate graft function; IL, interleukin; LIGHT, homologous to lymphotoxins, exhibits inducible expression, and competes with herpes simplex virus glycoprotein D for herpesvirus entry mediator, a receptor expressed by T lymphocytes; NMP, normothermic machine perfusion; proinflammatory cytokines: sCD40L, soluble CD40 ligand; PlGF, placental growth factor; PNF, primary nonfunction (never functioning graft 3 mo after transplantation); sFlt1, soluble Fms-like tyrosine kinase-1; Srage, soluble receptor for advanced glycation end-product; sST2, soluble suppression of tumorigenicity 2; TIE1, tyrosine kinase with immunoglobulin and epidermal growth factor homology domain 1; TIE2, tyrosine kinase with immunoglobulin and epidermal growth factor homology domain 2; TNFα, tumor necrosis factor alpha.