HitHunter® cAMP Assay for Small Molecules 90-0075SM2 from DiscoverX

Product Specs
ItemHitHunter® cAMP Assay for Small Molecules
CompanyDiscoverX
Price Supplier Page View Company Product Page
Catalog Number90-0075SM2
Quantity200/800 dp (96-well/384-well)
ApplicationsGPCR cAMP & Calcium Assays
Detection TargetcAMP
Research AreaGPCR cAMP & Calcium Assays

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DiscoveRx Corporation 42501 Albrae St.

Fremont, California 94538

United States

Phone: (510) 979-1415

Email: CustomerServiceDRX@eurofins.com

Company Profile

Website: http://www.discoverx.com

(1) GLP-2 and GIP acutely increase superior mesenteric artery blood flow in male rats, and the effect is independent of nitric oxide and vasoactive intestinal peptidePhysiol RepDecember 13, 2025Katrine D. Galsgaard, Bolette Hartmann, Mette M. Rosenkilde, Jens J. Holst, Lærke S. Gasbjerg, Charlotte M. Sørensenand GLP‐2 were custom‐made from Wuxi with a purity of >95%. The HitHunterTM cAMP XS assay (cat. no. 90‐0075SM2, DiscoveRx, Herlev, Denmark) was carried out according to the manufacturer's instructions. 2.4 Statistics To

(2) Steric control of signaling bias in the immunometabolic receptor GPR84bioRxivAugust 2, 2025Pinqi Wang, Xuan Zhang, Abdul-Akim Guseinov, Laura Jenkins, Carl von Hallerstein, Jonathan D. Colburn, Rowan Ives, Vincent B. Luscombe, Sara Marsango, Listiana Oktavia, et al.appropriately. cAMP levels were quantified using the HitHunter® cAMP Assay for Small Molecules (DiscoverX 90-0075SM2) according to the manufacturer’s protocol. Luminescence was measured 18 h post-assay using a PHERAstar® FS

(3) Kinetic insights into agonist-dependent signalling bias at the pro-inflammatory G-protein coupled receptor GPR84European Journal of PharmacologyOctober 5, 2023Vincent B. Luscombe, Luis Alberto Baena-López, Carole J.R. Bataille, Angela J. Russell, David R. Greavesand detection of cAMP was performed using the HitHunter® cAMP Assay for Small Molecules (DiscoverX 90-0075SM2 as per the manufacturer's instructions). Luminescence was measured 18–24 h after the final step on a

(4) A rare human variant that disrupts GPR10 signalling causes weight gain in miceNature communicationsMarch 15, 2023Talbot F, Feetham CH, Mokrosiński J, Lawler K, Keogh JM, Henning E, Mendes de Oliveira E, Ayinampudi V, Saeed S, Bonnefond A, et al.cAMP generation in GPR10 WT stably expressing cells was done using HitHunter® cAMP assay (DiscoverX, 90-0075SM) according to manufacturer’s protocol with modifications. 20,000 cells/well were seeded in a white poly-D-lysine

(5) MC3R links nutritional state to childhood growth and the timing of pubertyNatureNovember 18, 2021B. Y. H. Lam, A. Williamson, S. Finer, F. R. Day, J. A. Tadross, A. GonÃ§alves GonÃ§alves Soares, K. Wade, P. Sweeney, M. N. Bedenbaugh, D. T. Porter, et al.intracellular cAMP concentration using a luminescence-based HitHunter cAMP Assay for Small Molecules (DiscoverX 90– 0075SM25, Eurofins DiscoverX) and the Tecan Infinite M1000 Pro microplate reader. The cAMP standard curve was

(6) Lipolysis drives expression of the constitutively active receptor GPR3 to induce adipose thermogenesisCellJune 24, 2021Sveidahl Johansen O, Ma T, Hansen JB, Markussen LK, Schreiber R, Reverte-Salisa L, Dong H, Christensen DP, Sun W, Gnad T, et al.water before cAMP was measured using HitHunter® cAMP Assay for Small Molecules (Eurofins DiscoverX, #90-0075SM10) according to the manufacturer’s protocol. Luminescence was read on EnVision Multilabel Plate Reader

(7) Loss-of-function mutations in the melanocortin 4 receptor in a UK birth cohortNature MedicineJune 1, 2021Kaitlin H. Wade et al.concentration measurement using a luminescence based HitHunter cAMP Assay for small molecules (Cat# DiscoverX 90-0075SM25 Eurofins DiscoverX, Fremont, CA, USA) and a Tecan Spark 10M microplate reader. The baseline and maximal

(8) Paracrine signalling by cardiac calcitonin controls atrial fibrogenesis and arrhythmiaNatureNovember 1, 2020Moreira LM, Takawale A, Hulsurkar M, Menassa DA, Antanaviciute A, Lahiri SK, Mehta N, Evans N, Psarros C, Robinson P, et al.adenosine monophosphate (cAMP) was quantified using HitHunter cAMP Assay for 527 Small Molecules kit (#90-0075SM2, DiscoverX-Eurofins, USA); cAMP was measured in the 528 presence or absence of the selective inhibitor

(9) The orphan G protein-coupled receptor GPR139 is activated by the peptides: Adrenocorticotropic hormone (ACTH), α-, and β-melanocyte stimulating hormone (α-MSH, and β-MSH), and the conserved core motif HFRWNeurochemistry internationalJanuary 1, 2017Nøhr AC, Shehata MA, Hauser AS, Isberg V, Mokrosinski J, Andersen KB, Farooqi IS, Pedersen DS, Gloriam DE, Bräuner-Osborne H.expressed in COS7 cells using cAMP accumulation assay (HitHunter® cAMP assay, DiscoverX, Fremont, CA, USA, 90-0075SM). The COS-7 cell line was grown in Dulbecco's modified Eagle's medium (DMEM) with 1000 mg/l glucose

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HitHunter® cAMP Assay for Small Molecules from DiscoverX

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