Lipid Peroxidation Assay Kit (Cell-based) ab243377 from Abcam

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ItemLipid Peroxidation Assay Kit (Cell-based)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab243377
Quantity200 Test

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(1) Sulfatide Acts as a Regulatory Molecule Controlling β1 Integrin-STAT5 Signaling and BOLA2-Dependent Apoptotic Pathway in Breast Cancer CellsInt J Mol SciDecember 9, 2025Jaroslaw Suchanski, Weronika Woldanska, Adam Sciana, Barbara Suchanska, Leszek MoniakowskiNY, USA). The following day, lipid peroxidation was determined using a lipid peroxidation assay kit (ab243377, Abcam, Cambridge, UK) in cells treated with 50 µM H2O2 or 250 µM H2O2 (30 min). In brief, a ratiometric

(2) Iron overload-induced ferroptosis in CD8+ T cells leads to functional abnormalities that promote endometriosis progressionBMC MedOctober 27, 2025Shuang Wang, Le Xu, Xue Jiao, Xiaoyu Dong, Zhaoyang Zhong, Qianhui Ren, Xiaoxuan Liu, Ming Yuan, Guoyun Wangexamined using flow cytometry. Lipid peroxidation and ROS measurement 1X Lipid Peroxidation Sensor (ab243377, Abcam, UK) was added to the cells and incubated at 37 °C for 30 min in the dark. Cells were washed

(3) A chronic Pseudomonas aeruginosa mouse lung infection modeling the mucus obstruction, lung function, and inflammation of human cystic fibrosisInfection and ImmunityJuly 8, 2025Mylene Vaillancourt, Sheryl E. Fernandes, Diane Aguilar, Anna Clara Milesi Galdino, Peter Jorth8,000 rcf for 1 min. Pellets were resuspended in 100 µL of lipid peroxidation reagent 1:500 (Abcam cat# ab243377) and incubated for 30 min at 37°C and 5% CO2. Cells were washed with 1 mL 3% FBS/1× PBS buffer, spun

(4) NR4A1 suppresses breast cancer growth by repressing c-Fos-mediated lipid and redox dyshomeostasisExperimental & Molecular MedicineApril 1, 2025Cen Jiang, Youzhi Zhu, Junsi Zhang, Huaying Chen, Weiwei Li, Ruiwang Xie, Lingjun Kong, Ling Chen, Xiangjin Chen, Huifang Huang, et al.assay The level of lipid peroxidation was assessed using the Lipid Peroxidation Assay Kit (Abcam, cat. no. ab243377). In brief, cells were stained with 1× Lipid Peroxidation Reagent for 30 min in complete growth medium

(5) Effects of genetic ablation and pharmacological inhibition of HuR on gene expression, iron metabolism, and hormone levelsBMC BiolJanuary 23, 2025Nathalie Idlin, Sivakumar Krishnamoorthy, Magdalena Wolczyk, Mouad Fakhri, Michal Lechowski, Natalia Stec, Jacek Milek, Pratik Kumar Mandal, Jaroslaw Cendrowski, Christos Spanos, et al.using the PE channel. Lipid peroxidation was assessed with the Lipid Peroxidation Assay Kit (Abcam, ab243377) per kit instructions. Oxidized lipid levels were detected by flow cytometry by comparing FITC and PE

(6) Exosomal miR-375-3p mediated lipid metabolism, ferritinophagy and CoQ-dependent pathway contributes to the ferroptosis of keratinocyte in SJS/TENInt J Biol SciJanuary 20, 2025Chen Zhang, Pei Qiao, ChunYing Xiao, ZiPeng Cao, JiaoLing Chen, Hui Fang, JianKang Yang, ZeHua Kang, ErLe Dang, Shuai Shao, et al.peroxidation assay Lipid peroxidation levels were determined with a lipid peroxidation assay kit (ab243377, Abcam) according to the manufacturer's instructions. Briefly, human primary keratinocytes were seeded

(7) A chronic Pseudomonas aeruginosa mouse lung infection modeling the pathophysiology and inflammation of human cystic fibrosisbioRxivOctober 11, 2024Mylene Vaillancourt, Diane Aguilar, Sheryl E. Fernandes, Peter A. Jorth8000 rcf for 1 min. Pellets were resuspended in 100 μL of lipid peroxidation reagent 1:500 (Abcam cat# ab243377) and incubated for 30 min at 37°C and 5% CO2. Cells were washed with 1 mL 3% FBS/ 1X PBS buffer, spun

(8) Inhibition of ferroptosis rescues M2 macrophages and alleviates arthritis by suppressing the HMGB1/TLR4/STAT3 axis in M1 macrophagesRedox BiologySeptember 1, 2024Zhuan Feng, Feiyang Meng, Fei Huo, Yumeng Zhu, Yifei Qin, Yu Gui, Hai Zhang, Peng Lin, Qian He, Yong Li, et al.control cells. For lipid peroxidation, cells were incubated with a Lipid Peroxidation Sensor (Abcam, ab243377) in a humidified chamber at 37 °C with 5 % CO2 for 30 min, and changes in fluorescence from PE to FITC

(9) The atypical antidepressant tianeptine confers neuroprotection against oxygen–glucose deprivationEuropean Archives of Psychiatry and Clinical NeuroscienceJune 1, 2024Burcu Ersoy, Marie-Louise Herzog, Wen Pan, Simone Schilling, Matthias Endres, Ria Göttert, Golo D. Kronenberg, Karen Gertzratiometric fluorescent based lipid peroxidation assay was performed following the manufacturer’s guidelines (ab243377 Lipid Peroxidation Assay Kit, Abcam). Fluorescence intensities were measured using ImageJ software (NIH,

(10) Use of Optical Redox Imaging to Quantify Alveolar Macrophage Redox State in Infants: Proof of Concept Experiments in a Murine Model and Human Tracheal Aspirates SamplesAntioxidants (Basel)April 29, 2024He N. Xu, Diego Gonzalves, Jonathan H. Hoffman, Joseph A. Baur, Lin Z. Li, Erik A. Jensenperoxidation detection, the Cell-based Lipid Peroxidation Assay Kit (Abcam, Fremont, CA, USA, Cat. # ab243377) was used per the manufacturer’s instructions, where the green fluorescence was imaged with Ex 450–488

(11) MUC1-C is a target of salinomycin in inducing ferroptosis of cancer stem cellsCell death discoveryJanuary 5, 2024Daimon T, Bhattacharya A, Wang K, Haratake N, Nakashoji A, Ozawa H, Morimoto Y, Yamashita N, Kosaka T, Oya M, et al.assay Ratiometric measurement of lipid peroxidation was performed using the Lipid Peroxidation Assay Kit (ab243377; Abcam) according to the manufacturer’s instructions. Cells were analyzed by MACSQuant Analyzer 10 Flow Cytometer

(12) Effect of Photobiomodulation on Protein Kinase Cδ, Cytochrome C, and Mitochondria in U87 MG CellsCellsMay 22, 2023Viktória Pevná, Georges Wagnières, Daniel Jancura, Veronika Huntošováthe spectral range > 580 nm). Lipid peroxidation was visualized with a lipid peroxidation assay kit (ab243377, Abcam, Cambridge, UK) according to supplier protocol. MitoTracker Orange CMTMRos (1 µM, 15 min, ThermoFisher

(13) A compensatory RNase E variation increases Iron Piracy and Virulence in multidrug-resistant Pseudomonas aeruginosa during Macrophage infectionPLoS pathogensApril 1, 2023Vaillancourt M, Galdino ACM, Limsuwannarot SP, Celedonio D, Dimitrova E, Broerman M, Bresee C, Doi Y, Lee JS, Parks WC, et al.pellets were washed with 1 mL FC buffer (3% FBS in 1X PBS). The Lipid Peroxidation Assay Kit (Abcam cat# ab243377) was used and cells were stained according to the manufacturer’s instructions. Cells were then washed

(14) LCN2 secreted by tissue-infiltrating neutrophils induces the ferroptosis and wasting of adipose and muscle tissues in lung cancer cachexiaJournal of hematology & oncologyMarch 27, 2023Wang D, Li X, Jiao D, Cai Y, Qian L, Shen Y, Lu Y, Zhou Y, Fu B, Sun R, et al.analysis Adipocyte lipid ROS production was measured using the Lipid Peroxidation Assay Kit (Abcam, Cat#ab243377). Adipocytes purified from eWAT or iWAT were stained with the Lipid Peroxidation Sensor for 30 min at

(15) Isolation of adoptively transferred CD8(+) T cells in mouse tumor tissues for lipid peroxidation detectionSTAR protocolsMarch 17, 2023Xiao L, Yi Q.ice.Alternatives: if BODIPY™ 581/591 C11 is out of order, Lipid Peroxidation Assay Kit (Cell-based) (ab243377) from Abcam is a good choice. Data collection and analysis Timing: 1∼2 h This step illustrates how

(16) Uptake of Plasmodium chabaudi hemozoin drives Kupffer cell death and fuels superinfectionsSci RepNovember 17, 2022Isabella C. Hirako, MaÃsa Mota Antunes, Rafael Machado Rezende, NatÃ¡lia Satchiko Hojo-Souza, Maria Marta Figueiredo, Thomaz Dias, Helder Nakaya, Gustavo Batista Menezes, Ricardo Tostes Gazzinelliassay Lipid peroxidation in Kupffer cells was assessed by using lipid peroxidation assay kit (Abcam Ab243377). 1 × 106 of liver non-parenchymal cells were stained with antibodies specific for mouse CD11b (PE-Cy7,

(17) Glutamine Transporter SLC1A5 Regulates Ionizing Radiation-Derived Oxidative Damage and FerroptosisOxidative Medicine and Cellular LongevityOctober 10, 2022Zhuhui Yuan, Tong Liu, Xiao Huo, Hao Wang, Junjie Wang, Lixiang Xuewith 10 Gy at a dose rate of 6 Gy/min. Fresh media containing 0.1X BODIPY 581/591 C11 dye (Abcam, Cat# ab243377) was added to each well after the cells had been treated for 24 hours. The cells were rinsed with PBS

(18) AXL inhibition improves BRAF-targeted treatment in melanomaScientific reportsMarch 24, 2022Nyakas M, Fleten KG, Haugen MH, Engedal N, Sveen C, Farstad IN, Flørenes VA, Prasmickaite L, Mælandsmo GM, Seip K.treatments took place. Lipid peroxidation was evaluated by using Lipid peroxidation cell-based assay kit (ab243377, Abcam) according to the manufacturer`s instructions. The lipid peroxidation sensor supplemented samples

(19) Endothelial cell-derived pro-fibrotic factors increase TGF-β1 expression by smooth muscle cells in response to cycles of hypoxia-hyperoxiaBiochimica et biophysica acta. Molecular basis of diseaseJanuary 1, 2022Ismaeel A, Miserlis D, Papoutsi E, Haynatzki G, Bohannon WT, Smith RS, Eidson JL, Casale GP, Pipinos II, Koutakis P.,R&D Systems,DY6220-05 Assays,Assays,Assays MitoSOX Red,Thermo Fisher,M36008 Lipid Peroxidation Assay Kit (Cell-based),Abcam,Ab243377 Vybrant Cell Adhesion Assay Kit,Thermo Fisher,V13181

(20) CDC27-ODC1 Axis Promotes Metastasis, Accelerates Ferroptosis and Predicts Poor Prognosis in NeuroblastomaFrontiers in oncologyJanuary 1, 2022Qiu L, Zhou R, Luo Z, Wu J, Jiang H.detect the well-known lipid peroxidation marker of ferroptosis, using a Lipid Peroxidation Assay Kit (ab243377, Abcam, Cambridge, USA) according to the protocols listed in the official instructions. Reduced Glutathione

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Lipid Peroxidation Assay Kit (Cell-based) from Abcam

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Lipid Peroxidation Assay Kit (Cell-based) from Abcam

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