Diacylglycerol Assay Kit ab242293 from Abcam

Product Specs
ItemDiacylglycerol Assay Kit
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab242293
Quantity100 Test
Sample TypeCell Lysate

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) INSIG1/2 succination mediated by the moonlighting function of ADSL promotes lipogenesis and liver tumorigenesisNature CommunicationsMarch 15, 2026Yuran Duan, Shuo Wang, Jianyu Liu, Wenxing Qin, Yuli Shen, Yueru Hou, Xue Sun, Yanni Lin, Zhiqiang Hu, Bofei Dong, et al.antibodies were obtained from Santa Cruz Biotechnology. ATP-γ-S (ab138911) and Diacylglycerol assay kit (ab242293) were obtained from Abcam. 2SC (crb2005017) was purchased from Cambridge Research Biochemicals. [3H]25-hydroxycholesterol

(2) NAT10-mediated ac4C modification of Lipin1 mRNA contributes to the pathogenesis of PWMIJCI InsightAugust 8, 2025Xinyu Li, Meng Zhang, Yanan Liu, Chunjie Guo, Yiwei Liu, Lei Han, Zhaowei Feng, Xiue Wei, Ruiqin Yaonormalized based on the recorded values. DG was measured by using the Diacylglycerol Assay Kit (Abcam, ab242293) according to the manufacturer’s instructions. Brain tissue was homogenized and mixed with methanol,

(3) Functional regulation of macrophages by Ces1d-mediated lipid signaling in immunometabolismMolecular metabolismJuly 1, 2025Shao LJ, Elizondo F, Gao F, Habib R, Li X, Pham K, Ysaguirre J, Elizondo M, Shirazi S, Eckel-Mahan KL, et al.BMDM samples, BMDM conditional medium, and serum were quantified using the Diacylglycerol Assay Kit (ab242293, Abcam) according to the manufacturer's protocol. Briefly, serum and lysates from liver tissues and

(4) IDH status dictates oHSV mediated metabolic reprogramming affecting anti-tumor immunityNat CommunApril 24, 2025Upasana Sahu, Matthew P. Mullarkey, Sara A. Murphy, Joshua C. Anderson, Vasanta Putluri, Abu Hena Mostafa Kamal, Jun Hyoung Park, Tae Jin Lee, Alexander L. Ling, Benny A. Kaipparettu, et al.control. Diacylglycerol (DAG) assay Quantification of DAG was done using the DAG assay kit (Cat. No. ab242293, Abcam, USA) according to the manufacturer’s instructions. Briefly, 48 h post-treatment 107 cells/group

(5) Targeting the PTN/PTPRZ1-ROS Pathway to Promote Bone RegenerationBiomedicinesMarch 12, 2025Zhao K, Guo Y, He Y, Wu Y, Hu Z, Chi X, Deng X.TCS SP8). Diacylglycerol (DAG) levels in the samples were detected using a Diacylglycerol Assay Kit (Abcam, ab242293) according to the manufacturer’s protocol. 2.6. RNA Sequencing RNA was extracted from rBMSCs using

(6) TMEM135 deficiency improves hepatic steatosis by suppressing CD36 in a SIRT1-dependent mannerMolecular metabolismFebruary 1, 2025Chhetri A, Park C, Kim H, Manandhar L, Chuluunbaatar C, Hwang J, Wei X, Jang G, Chinbold B, Kwon HM, et al.before. 2.15 Diacylglycerol (DAG) measurement Intracellular DAG levels were determined using DAG assay kit (ab242293, Abcam, Cambridge, MA, USA) according to the manufacturer's instruction. Briefly, cells were harvested

(7) Metabolic dysfunction-associated steatotic liver disease is associated with effects on cerebral perfusion and white matter integrityHeliyonOctober 15, 2024Seidel F, Vreeken D, Custers E, Wiesmann M, Özsezen S, van Duyvenvoorde W, Caspers M, Menke A, Morrison MC, Verschuren L, et al.analysis, liver diacylglycerols (DAG) were quantified in the liver homogenates with DAG Assay Kit (ab242293, Abcam, Cambridge, UK) according to manufacturer's instructions. 2.4.3 Liver next-generation sequencing (NGS

(8) A protocol for measuring the activity of protein kinase C-delta in murine bone-marrow-derived dendritic cellsSTAR ProtocolsSeptember 20, 2024Seyedeh Mahdiye Mohati, Arash Mohammadi Matak, Stephanie Makdissi, Francesca Di Caraplasma membrane.Note: We followed and modified this method from the Diacylglycerol Assay Kit (Abcam AB242293-1001) the manufacturer’s protocol.Note: The Diacylglycerol Assay Kit suggests extracting lipids from

(9) Comparative membrane lipidomics of hepatocellular carcinoma cells reveals diacylglycerol and ceramide as key regulators of Wnt/β‐catenin signaling and tumor growthMolecular OncologyNovember 1, 2023Yagmur Azbazdar, Yeliz Demirci, Guillaume Heger, Dogac Ipekgil, Mustafa Karabicici, Gunes Ozhanisolation and determination of DAG content were performed according to manufacturer's instructions (ab242293, Abcam, MA, USA). Significance was tested using Student's t‐test. 2.11 Western blotting Cells were

(10) DGKB mediates radioresistance by regulating DGAT1-dependent lipotoxicity in glioblastomaCell reports. MedicineJanuary 17, 2023Kang H, Lee H, Kim K, Shin E, Kim B, Kang J, Kim B, Lee JS, Lee JM, Youn H, et al.System,Dako,K3468 DAG Kinase Activity Assay Kit,CELL BIOLABS,MET-5036 Diacylglycerol Assay Kit,Abcam,ab242293 Phosphatidic Acid Assay Kit,Abcam,ab273335 ATP Assay Kit,Abcam,ab83355 L-carnitine,Tokyo Chemical

(11) Detrimental effects of branched-chain amino acids in glucose tolerance can be attributed to valine induced glucotoxicity in skeletal muscleNutrition & DiabetesDecember 1, 2022Christopher A. Bishop, Tina Machate, Thorsten Henning, Janin Henkel, Gerhard PÃ¼schel, Daniela Weber, Tilman Grune, Susanne Klaus, Karolin Weitkunatafter extraction with 0.1 N NaOH. Quadricep DAGs were measured according to manufacturer’s protocol (ab242293, Abcam). Relative lipid content was quantified in randomly chosen microphotographs of H&E-stained liver

(12) Intervention with isoleucine or valine corrects hyperinsulinemia and reduces intrahepatic diacylglycerols, liver steatosis, and inflammation in Ldlr−/−.Leiden mice with manifest obesity‐associated NASHThe FASEB JournalAugust 1, 2022Eveline Gart, Wim van Duyvenvoorde, Martien P. M. Caspers, Nikki van Trigt, Jessica Snabel, Aswin Menke, Jaap Keijer, Kanita Salic, Martine C. Morrison, Robert Kleemann(DAG) content was quantified in crude liver homogenates according to the manufacturer's instructions (ab242293, Abcam, Cambridge, UK). In short, this assay extracts cellular lipids using methanol, chloroform, and

(13) Intervention with isoleucine or valine corrects hyperinsulinemia and reduces intrahepatic diacylglycerols, liver steatosis, and inflammation in Ldlr−/−.Leiden mice with manifest obesity‐associated NASHFASEB JJuly 13, 2022Eveline Gart, Wim van Duyvenvoorde, Martien P. M. Caspers, Nikki van Trigt, Jessica Snabel, Aswin Menke, Jaap Keijer, Kanita Salic, Martine C. Morrison, Robert Kleemann(DAG) content was quantified in crude liver homogenates according to the manufacturer's instructions (ab242293, Abcam, Cambridge, UK). In short, this assay extracts cellular lipids using methanol, chloroform, and

(14) Hepatocyte-specific PKCβ deficiency protects against high-fat diet-induced nonalcoholic hepatic steatosisMolecular metabolismFebruary 1, 2021Shu Y, Hassan F, Coppola V, Baskin KK, Han X, Mehta NK, Ostrowski MC, Mehta KD.[32P]ATP. The manufacturer's instructions for a commercially available DAG kit were followed (Abcam ab242293). The 32P-labeled phosphatidic acid was purified using chloroform/methanol/acetic acid (65:15:5, v/v)

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Diacylglycerol Assay Kit from Abcam

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Diacylglycerol Assay Kit from Abcam

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