Fig 1: Detailed phenotypic analyses of cancer-related alterations in peripheral immune cells in Lynch syndrome. Median marker expressions of CD197 on monocytes, mDCs and pDCs in each subject (A). Extended data including age-related analysis are presented in Additional File 2: Supplementary Figure S18. Plasma CCL19 and CCL21 levels in patients with untreated microsatellite-unstable cancer and age-matched healthy controls (B). R values of Pearson’s correlation analyses are also demonstrated. Extended data including baseline characteristics of this cohort can be found in Additional File 1: Supplementary Table S10 and Additional File 2: Supplementary Figure S19. Heatmap of median marker expressions and relative frequencies of each identified peripheral CD8+ T cell subclusters (C). UMAP representation of peripheral CD8+ T cell subclusters (D). UMAP representation of peripheral CD8+ T cells color-coded by CD38 (E) and HLA-DR (F) expressions. Extended data for further marker expressions can be found in Additional File 2: Supplementary Figure S20. Comparison of relative frequencies of CD8_T_10 subclusters between study groups (G). Extended data including analyses of further CD8+ T cell subclusters with altered relative frequencies between study groups and age-related analyses are presented in Additional File 2: Supplementary Figures S21-S22. Comparison of median marker expressions of CD194, CD28 and CD197 on CD8+ T cell subcluster CD8_T_10 between study groups (H). Heatmap of median marker expressions and relative frequencies of each identified peripheral monocyte subclusters (I). UMAP representation of peripheral monocyte subclusters (J). UMAP representation of peripheral monocytes color-coded by CD16 expression (K). Extended data for further marker expressions can be found in Additional File 2: Supplementary Figure S23. Comparison of relative frequencies of mono_5 subclusters between study groups (L). Extended data including age-related analysis are presented in Additional File 2: Supplementary Figure S24. On violin plots, dashed lines correspond to median values, while dotted lines border interquartile ranges. Asterisks correspond to statistical significance: p < 0.05*; < 0.01**; < 0.001***. Abbreviations: mDCs myeloid dendritic cells, pDCs plasmacytoid dendritic cells