Fig 1: FVII, FX and FXII are oxidized in trauma patients. FV (A), FX (B) and FXII (C) were immunoprecipitated using monoclonal antibodies against FV, FX and FXII from plasma of trauma patient (n = 5) and healthy controls (n = 4) as described in Methods. Purified FV, FX and FXII was analyzed by OxyBlot and immunoblotting using FV, FX or FXII – specific antibodies. Representative blots are displayed.