Description
Product Characteristics: The AssayMax™ Cyclophilin F (PPIF) ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of human PPIF in plasma, serum, and cell culture samples. This assay employs a quantitative sandwich enzyme immunoassay technique that measures human PPIF in 4 hours. A polyclonal antibody specific for human PPIF has been pre-coated onto a 96-well microplate with removable strips. PPIF in standards and samples is sandwiched by the immobilized antibody and the biotinylated polyclonal antibody specific for PPIF, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
Target Information: The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein is part of the mitochondrial permeability transition pore in the inner mitochondrial membrane. Activation of this pore is thought to be involved in the induction of apoptotic and necrotic cell death. [provided by RefSeq, Jul 2008]