Description
Product Characteristics: The AssayMax™ Canine RBP4 ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of canine RBP4 in plasma, serum, urine, and cell culture samples. This assay employs a quantitative sandwich enzyme immunoassay that measures canine RBP4 in less than 4 hours. A polyclonal antibody specific for canine RBP4 has been pre-coated onto a 96-well microplate with removable strips. Canine RBP4 in standards and samples is sandwiched by the immobilized antibody and biotinylated polyclonal antibody specific for canine RBP4, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
Target Information: This protein belongs to the lipocalin family and is the specific carrier for retinol (vitamin A alcohol) in the blood. It delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin which prevents its loss by filtration through the kidney glomeruli. A deficiency of vitamin A blocks secretion of the binding protein posttranslationally and results in defective delivery and supply to the epidermal cells. [provided by RefSeq, Jul 2008]