Description
Product Characteristics: The AssayMax™ Human HSF 1 ELISA (Enzyme-Linked Immunosorbent Assay) Kit is designed for detection of human HSF 1 in cell lysate and tissue extract samples. This assay employs a quantitative sandwich enzyme immunoassay technique that measures human HSF 1 in approximately 4 hours. A polyclonal antibody specific for human HSF 1 has been pre-coated onto a 96-well microplate with removable strips. HSF 1 in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for human HSF 1, which is recognized by a streptavidin- peroxidase (SP) conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
Target Information: The product of this gene is a heat-shock transcription factor. Transcription of heat-shock genes is rapidly induced after temperature stress. Hsp90, by itself and/or associated with multichaperone complexes, is a major repressor of this gene. [provided by RefSeq, Jul 2008]