Description
Product Characteristics: The AssayMax™ Human ADAMTS13 ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for detection of ADAMTS13 in human plasma, serum, saliva, CSF, and cell culture samples. This assay employs a quantitative sandwich enzyme immunoassay technique that measures ADAMTS13 in 4 hours. A polyclonal antibody specific for ADAMTS13 has been pre-coated onto a 96-well microplate with removable strips. ADAMTS13 in standards and samples is sandwiched by the immobilized antibody and biotinylated polyclonal antibody specific for ADAMTS13, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
Target Information: This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motif) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The enzyme encoded by this gene is the von Willebrand Factor (vWF)-cleaving protease, which is responsible for cleaving at the site of Tyr842-Met843 of the vWF molecule. A deficiency of this enzyme is associated with thrombotic thrombocytopenic purpura. Alternative splicing of this gene generates multiple transcript variants encoding different isoforms. [provided by RefSeq, Nov 2008]