Description
The Progesterone ELISA quantitates progesterone in urine, dried fecal extracts or cell culture medium.
Principle of the method
The Progesterone Competitive ELISA research-use-only kit is designed to quantitatively measure Progesterone present in extracted dried fecal samples, urine, and tissue culture media samples. A progesterone standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture mouse antibodies. A progesterone-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a monoclonal antibody to progesterone to each well. After a 2-hour incubation the plate is washed and substrate is added. The substrate reacts with the bound progesterone-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring at 450 nm.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation