Description
The Aldosterone Competitive ELISA quantitates aldosterone in extracted serum, plasma, urine, dried fecal extracts or cell culture medium.
Principle of the method
The Aldosterone Competitive ELISA Kit research-use-only kit is designed to quantitatively measure aldosterone present in extracted serum and plasma, or in urine, extracted dried fecal samples, and tissue culture media samples. An aldosterone stock solution is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into the provided plate coated with a capture antibody. An aldosterone-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to aldosterone to each well. After incubation, the plate is washed and substrate is added. The substrate reacts with the bound aldosterone-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring at 450 nm.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation