Description
For Quantitation of Leached Protein A in Bioprocess Samples
--Quantitation of recombinant Protein A constructs and MabSelect SuRe™
--Sensitivity down to 100 pg/mL
--Automated detection with minimal hands-on time
--Analyze 96 samples in under 2 hours on the Octet® RH96 system
The Ready BLI Detection Kit - Residual Protein A (RPA) applies the principles of Bio-Layer Interferometry (BLI) to enable sensitive, accurate quantitation of leached Protein A in bioprocess samples while maintaining a simple workflow. The kit utilizes a validated sample treatment method for dissociating Protein A from antibodies that does not require boiling, neutralization, or centrifugation steps.
Dip and Read RPA biosensors with pre-immobilized chicken anti-Protein A antibody are sequentially dipped into treated samples, buffers, and detection antibody arrayed in 96-well microplates to create a microscale immunoassay on the biosensor tip. Signal detection occurs in real time at the secondary antibody step without need for conjugates or enzymatic reactions