Description
Intended Uses: This sandwich kit is for the accurate quantitative detection of Mouse Free Prostate Specific Antigen (also known as FPSA) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse FPSA antibody. FPSA present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse FPSA Antibody is added and binds to FPSA in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated FPSA antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse FPSA. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm