Description
Principle of the Assay: MBS Human LBP ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human LBPin Cell Culture Supernates, Serum, Plasma. This assay employs an antibody specific for Human LBPcoated on a 96-well plate. Standards and samples are pipetted into the wells and LBPpresent in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human LBPantibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of LBPbound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Background: Lipopolysaccharide binding protein is a protein that in humans is encoded by theLBP gene. This gene is mapped to 20q11.23. LBP is a soluble acute-phase protein that binds to bacterial lipopolysaccharide(or LPS) to elicit immune responses by presenting the LPS to important cell surface pattern recognition receptors called CD14 and TLR4. It is present in the cerebrospinal fluid of patients with pneumococcal meningitis. The protein encoded by this gene is involved in the acute-phase immunologic response to gram-negative bacterial infections. LBP is made in the liver during the acute phase of infections and is thought to function as a carrier for LPS and to help control LPS-dependent monocyte responses