Description
Intended Uses: This sandwich kit is for the accurate quantitative detection of Mouse Dickkopf Related Protein 2 (also known as DKK2) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse DKK2 antibody. DKK2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse DKK2 Antibody is added and binds to DKK2 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated DKK2 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse DKK2. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm