Description
Intended Uses: This sandwich kit is for the accurate quantitative detection of Mouse Total Cholesterol (also known as TC) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Mouse TC antibody. TC present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse TC Antibody is added and binds to TC in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated TC antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse TC. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm