Description
Intended Uses: This sandwich kit is for the accurate quantitative detection of human Proto-oncogene C-Jun (also known as C-Jun) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human C-Jun antibody. C-Jun present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human C-Jun Antibody is added and binds to C-Jun in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated C-Jun antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human C-Jun. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm