Description
Intended Uses: This sandwich kit is for the accurate quantitative detection of human Alpha 2-Antiplasmin (also known as A2-AP) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human A2-AP antibody. A2-AP present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human A2-AP Antibody is added and binds to A2-AP in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated A2-AP antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human A2-AP. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm