Fig 1: Boxplots for the plasma concentrations of BMP4, Noggin, and inflammatory cytokines before surgery (T1), at the end of the surgery (T2), and 24 hours after surgery (T3). The results of the Dunn’s multiple comparison test are presented in each figure. *Indicate a P-value <0.05; **Indicate a P-value <0.01.
Fig 2: The decrease of BMP circulating concentration and the deficiency of BMP/Smad signaling in IDD patients. (A-F) Serum concentrations of BMPs. Circulating levels of BMP1 (A), BMP2 (B), BMP3 (C), BMP4 (D), BMP5 (E), and BMP7 (F) were measured in serum samples obtained from healthy controls (n = 20) and IDD patients (n = 20). *P < 0.05 and **P < 0.01. (G) The protein levels of BMP/Smad signaling molecules. Total cell extracts from the IVDs in one control and different Pfirrmann grades (I-IV) were subjected to immunoblots to examine the protein levels of BMPR1a, BMPR1b, BMPR2, Smad1/5/8, pSSmad1/5/8, Smad4, and β-Actin (loading control).
Fig 3: Correlations among plasma concentrations of BMP4, Noggin, and inflammatory cytokines. The Spearman’s rank correlation coefficient (r) and the correlation test’s significance level (P) are presented in each figure. The solid lines represent the fitted linear regression trendline.
Fig 4: High glucose promoted the metastasis of CRC validated in vivo studies(A) Protocol for high-fat diet (HFD) and STZ-induced diabetes mouse models and liver metastasis model(B) Representative image of the liver of mice with diabetes (DM + CA group) and without diabetes (CA group)(C) BMP4 and E-cadherin, N-cadherin, Vimentin protein levels in formalin-fixed, paraffin-embedded tumors as detected by IHC.
Fig 5: High glucose promoted the proliferation and metastasis of CRC cells(A) Glucose consumption was measured in different types of CRC cell lines by glucose assay kit, *P < 0.05, ***P < 0.001(B) The result of glucose consumption was rectified by cell viability measured by CCK-8 kit (OD 450), *P < 0.05, ***P < 0.001(C) Protein of BMP4 expression examined by western blotting in different types of CRC cell lines(D, E) Treated with high glucose(50mM) 48 h could upregulate the expression of BMP4 measured by western blotting(D) and qRT-PCR(E), *P < 0.05(F) Cell viability of SW1116 and MC38 cultivated by high glucose(50mM) and Noggin(200nM) for 48 h assessed by CCK-8kit, *P < 0.05, ***P < 0.001(G) Transwell assays were performed to examine the potential migration of SW1116 and MC38 cells treated with or without high glucose(50mM) and Noggin(200nM).
Supplier Page from Abcam for Human BMP-4 ELISA Kit