Fig 1: Analysis of the levels of proteins involved in stemness in the DMEM(LG) and DMEM(HG) groups. In passage 4 cells, MeSCs from donors No. 1 to 4 and No. 6 did not significantly differ in the levels of Nanog (a), Oct4 (b), Sox2 (c), NESTIN (d) or Notch-1 (e) between the DMEM(LG) and DMEM(HG) groups. Relative expression levels were calculated as the relative change from the DMEM(HG) group. The test was performed on the cells from all the six donors. The experiment was performed in triplicate, and the mean value of every sample was used for group comparisons. Group comparisons were performed using the paired t-test. n = 5. The levels of Nanog (f), Oct4 (g), Sox2 (h), NESTIN (i) and Notch-1 (j) were significantly increased in the DMEM(LG) group of MeSCs from donor No. 5. Relative expression levels were calculated as the relative change from the DMEM(HG) group. The experiment was performed six times for each donor samples, and the mean value of every sample was used for group comparisons. Group comparisons were performed using the Mann–Whitney non-parametric. n = 6.