Fig 1: Decellularized lymph node characterization and ECM protein preservation. (A) Glycosaminoglycan (GAG) concentration in control and decellularized lymph nodes (n = 3). GAG concentration was measured using Abcam’s colorimetric Total Glycosaminoglycans kit (ab289842). (B) Total collagen concentration in control and decellularized lymph nodes (n = 4). Collagen concentration was measured using Abcam’s Total Collagen Assay Kit (Perchlorate-Free) (ab222942). (C) Elasticity measured by Atomic Force Microscopy in control and decellularized lymph node sections (n = 6–10). (D) Representative immunofluorescence images of control and decellularized lymph node sections stained for DAPI (Nuclei, gray), and ECM proteins Collagen IV (green), Fibronectin (magenta), and Pan-Laminin (cyan) (scale bar = 100 μm). Data represented as mean ± standard deviation. P-values were determined by unpaired, student’s t-test. (ns, not significant, p > 0.05).