Fig 1: Enhanced inflammatory response in CBLB502-pretreated mice after PHx. Serum TNF-a, IL-6, and G-CSF (a), and TGF-a and HGF (b) in CBLB502 treated mice at the indicated times. Serum TNF-a, IL-6, and G-CSF (c), and TGF-a and HGF (d) were measured in regenerating PBS and CBLB502 treated mouse livers after PHx. e Relative mRNA levels of hepatic c-Fos, c-Myc, c-Jun, TNF-a, and IL-6 in PBS and CBLB502 treated mice after PHx. f Cell counts of liver MNCs, neutrophils, KCs, and recruited macrophages in PBS and CBLB502 treated mice after sham surgery or PHx. Panel e shows means ± SD. Data are representative of three independent experiments. Panels a-d, f show means ± SEM. Data are derived from two or three independent experiments with similar results. n = 3–7. N.D., not detected. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001
Fig 2: ER stress dampens the epithelium repair function of LR-MSC. A Schematic illustration of transwell coculture of MLE-12 and LR-MSC. B, C show the apoptosis level of cocultured MEL-12 with LR-MSC. D The concentration of HGF, KGF, and TGFß1 in the coculture medium was evaluated by ELISA. E Transwell migration assay was performed to compare the migratory capacity of TM-pretreated LR-MSC. The left panel shows the counts of migrated LR-MSC by Giemsa staining
Fig 3: TLR5 deficiency suppresses hepatocyte priming in PHx-induced liver regeneration. a Relative mRNA levels of hepatic c-fos, c-myc, and c-jun in WT and Tlr5-/- mice at the indicated times after PHx. b Serum TNF-a, IL-6, TGF-a, and HGF were measured in regenerating WT and Tlr5-/- mouse livers. c Quantification of hepatic TNF-a, IL-6, TGF-a, and HGF mRNA expression in WT and Tlr5-/- mice at the indicated times after PHx. d Western blot analysis of the indicated target proteins in WT and Tlr5-/- mouse livers after PHx, with ß-actin as the loading control. Panels a and c show means ± SD. Results are representative of three independent experiments. Panel b shows means ± SEM. Data are derived from two or three independent experiments with similar results. n = 4–6. Western blot data in panel d are representative of three independent experiments. N.D., not detected. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001
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