Fig 1: Correlation between relative difference of serum biomarkers and EIT extracted parameters for patients with normal and abnormal concentration of S100B before surgery. (A) The heatmap of correlation coefficient between relative difference of S100B protein and EIT extracted information. Significant correlations can be seen in group of CT1-S100B > 105 pg/mL. (B) Correlation of ΔARVHCA and relative difference of S100B protein between T4 and T1 for patients with CT1-S100B > 105 pg/mL. (C) The correlation of kHCA and relative difference of S100B protein between T4 and T1 for patients with CT1-S100B > 105 pg/mL. (D) The correlation of MRAIabs and relative difference of S100B protein between T4 and T1 for patients with CT1-S100B > 105 pg/mL. T2–T1 relative difference between after and before surgery, T3–T1 relative difference between 12 h after and before surgery, T4–T1 relative difference between 24 h after and before surgery, T5–T1 relative difference between 48 h after and before surgery, HCA hypothermic circulatory arrest, ΔARVHCA the difference of average resistivity value before and after HCA phase, kHCA the slope of electrical impedance during HCA phase, MRAIabs maximum of the absolute value of resistivity asymmetric index, TARIabs absolute value of time integral of resistivity asymmetric index, TARVHP time integral of electrical impedance for half flow of perfusion; *means p < 0.05.
Fig 2: Visualization of S100B protein with the “S100 domain” highlighted in red and the “EF-hand domain” in green (A). Representation of the hydrophobic regions of S100B scaled from white (polar) to red (hydrophobic) (B), with the visualization of the aminoacidic sequence of the S100 domain (C). The three-dimensional “knock-fist“ structure of S100B (D).
Fig 3: The standard curve of a representative experiment (Abcam, ab234573, Cambridge, UK) and the location of a durian fruit extract regarding the concentration of S100B-like material.
Fig 4: Correlation between relative difference of serum biomarkers and EIT extracted parameters for patients with normal and abnormal concentration of TAU before surgery. (A) Heatmap of correlation coefficient between relative difference of TAU protein and EIT extracted information. Significant correlations can be seen in group of CT1-TAU > 115 pg/mL. (B) The correlation of ΔARVHCA and relative difference of TAU protein between T2 and T1 for patients with CT1-TAU > 115 pg/mL. (C) The correlation of kHCA and relative difference of TAU protein between T2 and T1 for patients with CT1-TAU > 115 pg/mL. (D) The correlation of TARVHP and relative difference of TAU protein between T2 and T1 for patients with CT1-S100B > 115 pg/mL. T2–T1 relative difference between after and before surgery, T3–T1 relative difference between 12 h after and before surgery, T4–T1 relative difference between 24 h after and before surgery, T5–T1 relative difference between 48 h after and before surgery, HCA hypothermic circulatory arrest, ΔARVHCA the difference of average resistivity value before and after HCA phase, kHCA the slope of electrical impedance during HCA phase, MRAIabs maximum of the absolute value of resistivity asymmetric index, TARIabs absolute value of time integral of resistivity asymmetric index, TARVHP time integral of electrical impedance for half flow of perfusion; **means p < 0.01.
Fig 5: Comparison between the S100B phylogenetic tree and the NCBI taxonomy tree. The phylogenetic tree derived from S100B analysis output corresponds to the NCBI taxonomy classification.
Supplier Page from Abcam for S100B ELISA Kit