Fig 1: Determination of pyroptosis-related factors in the brains of mice infected with RABV: (A–D) Determination of Gsdmd, Nlrp3, Casp1, and IL-1β in the brains of mice infected with RT-qPCR. (E) Western blot assay was used for detecting the expressions of the RABV N, Gsdmd, and NLRP3 proteins in all groups. (F–H) Quantification of the RABV N, Gsdmd, and NLRP3 proteins based on the Western blot assay. (I) ELISA for detection of the IL-1β protein. Asterisks indicate the statistical significance as follows: *, p < 0.05; **, p < 0.01.
Fig 2: Pyroptosis occurred in dissociated neurons of murine brains infected with RABV: (A) The flowchart of neuronal cells’ dissociation from the brains of mice inoculated intracerebrally with DMEM, rRC-HL, GX074, and CVS-24. The purity and morphology of the dissociated neuronal cells were assessed by means of IFA (NeuN, red; DAPI, blue, Scale bar = 100 μm). (B) The weight of the collected murine brains (n = 12). (C) Numbers of neuronal cells isolated from the brains of mice infected with RABV. (D) Detection of the neuronal cell survival rate (%) was conducted with trypan blue. (E–H) Determination of pyroptosis-related factors in neuronal cells by means of RT-qPCR. (I) Gsdmd concentration of neuronal cells dissociated from the brains of mice infected with RABV. Asterisks indicate the statistical significance as follows: *, p < 0.05; **, p < 0.01; ***, p < 0.001.
Fig 3: Protein–protein interaction (PPI) network: (A) The 62 proteins that could interact with at least 9 proteins were identified as the hub proteins based on the PPI network (The proteins of interest is highlighted in red). (B) The PPI networks of the Gsdmd signaling pathway. (C) The PPI networks of Tnf, IL-6, IL-10, and chemokines.
Supplier Page from Abcam for Mouse GSDMD ELISA Kit