Fig 1: Salivary proteins and vitamins during the clinical course of recurrent aphthous stomatitis. Verification of proteomic data points. (A) Western blotting showed higher CA1 protein levels during the ulcerative and remission stages. Ponceau red were used as loading control. (B) ELISA showed a higher amount of HBB protein in ulcerative and remission stages. (C) Samples from patients with recurrent aphthous stomatitis show lower levels of folate (vitamin B9) and vitamin B12, but without statistically significant differences. Data are represented by mean ± SD (Student’s t-test, different letters indicate statistically difference at p value = 0.05). CA1, carbonic anhydrase 1; SLURP1, secreted Ly-6/uPAR-related protein 1; HBB, hemoglobin subunit beta; CST1, cystatin-SN; A2M, alpha-2-macroglobulin.
Fig 2: As3+ exposure decreases hemoglobin (Hb) production during the erythroid development of CD34+-HPCs. Human bone marrow CD34+-HPCs were cultured in erythroid expansion medium for 6, 8, 11, 15 or 16 (15/16) and 19 or 23 (19/23) days in the presence of 0, 100 nM, or 500 nM As3+. Cell pellets collected at the indicated time points were imaged, and then lysed for cytoplasmic protein extraction. The cell lysates with a total protein concentration of 100 ng/mL were used for quantification of Hb using the Human HBB SimpleStep ELISA Kit (ab235654, Abcam). (a) Relative Hb levels at the different time points tested. Relative Hb levels were calculated as described in the methods section. (b) Representative images of cell pellets from control, 100 nM, and 500 nM As3+ on days 11 and 23, which were from same donor (Donor 3). Data are expressed as summarized mean ± SD. *(p < 0.05) in one-way ANOVA, followed by Tukey’s post hoc test compared between groups.
Supplier Page from Abcam for Human HBB ELISA Kit