DAPI Staining Solution from Abcam

Product Specs
ItemDAPI Staining Solution
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab228549
Quantity2 mL
Sample TypeAdherent cells, Suspension cells
TypeStain

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Six-month evaluation of normal mouse brain side effects: Comparing FLASH and conventional proton partial brain irradiationClin Transl Radiat OncolMay 9, 2026Manuel Bernabei, Sindi Nexhipi, Elisabeth Bodenstein, Felix Horst, Armin Lühr, Jörg Pawelke, Moritz Schneider, Michael Schürer, Roman Schwarz, Antje Dietrich, et al.studying microglial morphology. All slides were counterstained with DAPI (4′,6-diamidino-2-phenylin-dole, ab228549, Abcam, Cambridge, UK) for nuclei detection. Whole slide imaging was performed using the AxioScan.Z1

(2) Imaging mass cytometry of the immune microenvironment in alveolar echinococcosisFront Cell Infect MicrobiolMay 8, 2026Yanyan Ma, Yaogang Zhang, Tao Zhang, LingQing Zhang, Zhixin Wang, Zihan Yang, Xinjian Guo, Jingqi Han, Jing Hou, Meiyuan Tian, et al.(Goat Anti-Rabbit IgG H&L, Abcam, ab205718, 1:20000). The nuclei were stained with DAPI Staining Solution (Abcam, ab228549), antifade mounting medium was added, and the slides were sealed. Images were acquired using a TG TissueFAXS

(3) Phaeochromocytomas and paragangliomas harbour tumour-initiating SOX2+ stem cellsEndocr Relat CancerApril 22, 2026Yasmine Kemkem, Mark Quinn, Bence Kövér, Alice Santambrogio, James Kaufman-Cook, Olivia Sherwin, Laura D Scriba, Dimitria Brempou, Miriam Vazquez Segoviano, Dylan Cameron, et al.fluorophore-conjugated antibodies (Goat anti-mouse594 (CAT#ab150116, Abcam, UK, 1:500) and DAPI (CAT# ab228549, Abcam, UK, 1:5,000) in 1% donkey serum/blocking buffer. Cryosections were air-dried and washed in PBS.

(4) PFKFB4 promotes M2 polarization of tumor-associated macrophages through aerobic glycolysis-mediated modification of histone H3K18 lactylation in hepatocellular carcinomaCancer & MetabolismApril 15, 2026Chao Gan, Dan Wu, Yue Yuan, Lingling Li, Jia Bai, Yanmei Xu, Haihong Lvlight for 1 h. Cell nuclei (blue) were stained with 4’,6-diamidino-2-phenylindole staining solution (ab228549, Abcam). Sections were sealed using an anti-fluorescence quenching sealing agent (A598329, Aladdin Biochemical

(5) Programmable Lipid Functionalization of Nucleic Acid Nanoparticles Modulates Liver Cell-Type TargetingACS Applied Materials & InterfacesApril 8, 2026Hyun Min Kim, Marjan Omer, Grant A. Knappe, Patrick McMullen, Duy An Le, Ashwin Pasupathy, Daniel G. Anderson, Mark BatheE (#MAB41444-SP) were purchased from R&D Systems. Pitstop 2 (ab120687) and DAPI staining solution (ab228549) were purchased from Abcam. Twelve well chamber removable microscopy slides (#81201) were purchased

(6) TGFβ1-loaded extracellular matrix hydrogels promote skeletal muscle stem cell regeneration via m6A-mediated integrin–ERK signalingJournal of Translational MedicineApril 6, 2026Menghai Zhu, Peng Zou, Gang Chen, Chong Lian, Benggang Qingoat anti-rabbit IgG H&L (Alexa Fluor® 488) (1:1000 dilution, ab150077, Abcam) were used. Cell nuclei were counterstained with DAPI (ab228549, Abcam, USA). Cell images were obtained by an AxioVision 4.8 camera and an Axio Observer Z1 inverted

(7) Sacubitril/Valsartan Ameliorates Inflammation and Oxidative Stress in Hypertensive Heart Disease by Upregulating CAMKK2 Protein and Modulating the AMPK / AKT / GSK ‐3β AxisThe Kaohsiung Journal of Medical SciencesApril 1, 2026Yan‐Jun Yang, Jiu‐Sheng Lifluorescein, while total nuclei were stained with 4′, 6‐diamidino‐2‐phenylindole staining solution (DAPI, ab228549, Abcam). The apoptosis rate was determined by the ratio of TUNEL‐positive to DAPI‐stained nuclei. For

(8) TNF inhibits SARS-CoV-2 induced cell-cell fusion through activating the SDC4-RhoA signaling to promote actin bundles formationCell InsightApril 1, 2026Dong Duan, Xu Zheng, Yanqiu Zhou, Mengmeng Cui, Yunyi Li, Xiaoxian Cui, Yuying Yang, Min Chen, Huanyu Wu, Xin Chen, et al.(Invitrogen, A-21424, 1: 400) and goat anti-rabbit IgG-647 (Invitrogen, A-21236, 1: 400), DAPI (Abcam, ab228549, 1:2 000) and antifade mounting medium (vector labs, H-1400-10). Pam3CSK4 (Invivogen, tlrl-pms), TPCA1

(9) Anti-Cancer Effect of a New 5-FU Derivative Containing Triazole-Bearing Mannose (5-FUD-MAN) Against Human Breast Cancer Cells Through LC3B-Mediated Cell DeathJ Biochem Mol ToxicolMarch 29, 2026Ebru Şanci, Azada Aliyeva, Buket Bakan, Mustafa Özkaraca, Erkan Halay, Kadir Ay, Tamer Karayildirim, N. Ulku Karabay YavasogluCells were then incubated for 1 h at room temperature in the dark. Finally, DAPI (Abcam, Catalog no. ab228549) was applied to the washed cells and examination was performed by using fluorescence microscopy (Zeiss

(10) Gypenoside XLIX ameliorates diabetic retinopathy by downregulating prostaglandin-endoperoxide synthase 2 in retinal pigment epithelium cells to inhibit ferroptosis and preserve tight junction integrityFrontiers in PharmacologyMarch 23, 2026Jiayi Gu, Manhui Zhu, Lele Li, Xin Cao, Xiaoli Yu, Xiaobo Huang, Lili Huang, Qi Cai, Yan Zhu, Wendie Li, et al.United States). Anti-ZO-1 (1:100; Cat# ab221547) and 4′,6-diamidino-2-phenylindole (DAPI; 1:5,000; Cat# ab228549) from Abcam (Cambridge, United Kingdom). 2.3 Animal grouping and treatment Male C57BL/6 mice (6–8

(11) Low, plasma level‑informed native curcumin concentrations fail to induce cell death in human lung and colorectal cancer cellsPharm BiolMarch 20, 2026Ilma Imtiaz, Janet Schloss, Andrea BugarcicLI-COR Biosciences, while goat anti-rabbit Alexa Fluor® 488 (ab150081) and stains, including DAPI (ab228549) and Rhodamine Phalloidin (ab235138), were purchased from Abcam. Staurosporine (S5921), Triton X-100

(12) Targeting cardiomyocyte-derived extracellular vesicle miR-574-5p protects against cognitive impairment induced by ischemia–reperfusionBMC MedicineMarch 19, 2026Erya Chen, Xiaoyu Zhu, Haiqing Chang, Qi Li, Zhenkun Zhao, Changteng Zhang, Yu Cao, Yajing Wang, Xinliang Ma, Tao Zhu, et al.antibody at room temperature for 1–2 h. Next, the nuclei were stained with DAPI (Abcam, Cambridge, UK, no. ab228549) for 30 min at room temperature. Finally, the tissue sections were covered with coverslips after using

(13) In Vitro Assessment of Ventricular Catheters with a Multilayered Fibrous Web to Prevent Cellular OcclusionACS Biomaterials Science & EngineeringMarch 9, 2026Seunghyun Lee, Amirhossein Shahriari, Gio Jison, Noah Ramos, Sora Sato, Celine Tran, Leandro Castaneyra-Ruiz, Michael MuhonenA11034) was applied for 1 h. Finally, cells were stained with DAPI (4′,6-diamidino-2-phenylindole, ab228549, Abcam) (1:5000 in PBS) for nuclear labeling. ChPE cell cultures were labeled with antitransthyretin

(14) Age-mimicking hydrogel stiffness recapitulates the mechanical niche of the hippocampus to regulate neural stem cell senescenceMater Today BioMarch 3, 2026Luyao Guo, Longjiao Ge, Yong Li, Shouye Wang, Huitong Li, Xiaoyu Wang, Weiliang Qian, Yu Zhang, Liuhanhui Guo, Luxuan Guo, et al.(Jackson, 711-165-152, 1:1000), donkey anti-rat Cy3 (Jackson, 712-165-153, 1:1000) and DAPI (Abcam, ab228549, 1:1000). After removing the secondary antibodies and washing the sections three times with PBS, the

(15) Retrotransposon activation during spermatogenesis achieves massive ecDNA biogenesis, but rare integrationGenes & DevelopmentMarch 2, 2026Lauren Tracy, Yao Chen, Zz Zhao Zhangroom temperature. Then samples were washed three more times in PBST, with DAPI (1:1000, Abcam Cat. # ab228549) in the second wash step. Finally, the samples were mounted with Vectashield Mounting Medium. Images

(16) Extracellular vesicles from stem cells rescue cellular phenotypes and behavioral deficits in SHANK3-associated ASD neuronal and mouse modelsCell Death & DiseaseFebruary 22, 2026Ashwani Choudhary, Idan Rosh, Yara Hussein, Shai Netser, Aviram Shemen, Taghreed Suliman, Wote Amelo Rike, Lilach Simchi, Boris Shklyar, Ahmad Abu-Akel, et al.overnight at 4 °C. The next day, the coverslips were washed in DPBS and incubated with DAPI (Abcam, ab228549, 1:2500) and the corresponding secondary antibodies for 60 min. at room temperature. Then, the coverslips

(17) SNCA triplication disrupts proteostasis and extracellular architecture prior to neurodegeneration in human midbrain organoidsnpj Parkinson's DiseaseFebruary 16, 2026Elpida Statoulla, Maria Zafeiri, Kleanthi Chalkiadaki, Georgia Voudouri, Karmel S. Gkika, Christos Papazoglou, Thomas M. Durcan, Arkady Khoutorsky, Seyed Mehdi Jafarnejad, Sarah Maguire, et al.detailed list of antibodies can be found in Sup. Data 4. DRAQ5 (Abcam, #ab108410) or DAPI (Abcam, #ab228549) were used for nuclear staining. Imaging was performed using a LEICA SP5 and a NIKON A1R HD confocal

(18) Cell jamming transition is regulated by mitochondrial pyruvate transport and endocytosisbioRxivFebruary 13, 2026Alexandra Bermudez, Zoe Latham, Johnny Diaz, Weihong Yan, Jerry Chen, Dapeng Bi, Andrew Goldstein, Jimmy K Hu, Neil Y.C. Linanti-rabbit Alexa Fluor 647 (Invitrogen, A21042 and 4414S, respectively)—together with DAPI (Abcam, ab228549) and either phalloidin (Invitrogen, A12379) or actin stain (Invitrogen, R37110). Microscopy and Live-cell Imaging

(19) Obesity Reprogrammes Adipose Extracellular Vesicles to Induce Muscle Atrophy via miR-150-5p-Mediated Transcriptional SilencingJ Cachexia Sarcopenia MuscleFebruary 3, 2026Joshua M. J. Price, Michael Macleod, Thomas Nicholson, Caitlin M. Ditchfield, Bethy Airstone, Natalie Lachlan-Jiraskova, Edward T. Davis, Kostas Tsintzas, Simon W. Jonesat RT. Wells were then washed with PBS, after which DAPI staining solution (1:5000 in PBS, Abcam, #ab228549) was added for 5 min in the dark. Finally, wells were washed again with PBS, before Fluoromount Aqueous Mounting Medium

(20) Astrocytogenic bidirectional plasticity at spinal nociceptive synapses regulates acute nociceptive processingPainFebruary 1, 2026Sibel Ada, Laura Klinger, Hannah L. Teuchmann, Valeria Mussetto, Viktoria Hadschieff, Mira T. Kronschläger, Anna S.M. Siegert, Lidia Trofimova, Raphael Holzinger, Danijela Kurija, et al.×1 PBS (3 × 10 minutes) and incubated in ×1 PBS + 0.2% 40,6-diamidino-2-phenylindole (DAPI, Abcam, ab228549), protected from light, for 60 minutes. Slices were again washed with ×1 PBS (2 × 5 minutes) and mounted

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(1)

6/17/2020This DAPI Binds Strongly to Nuclei Samantha LanjewarIt stains nuclei well and is easy to use.

DAPI Staining Solution from Abcam

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DAPI Staining Solution from Abcam

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