DAPI Staining Solution from Abcam

Product Specs
ItemDAPI Staining Solution
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab228549
Quantity2 mL
Sample TypeAdherent cells, Suspension cells
TypeStain

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(141) Genomic and epigenomic basis of breast invasive lobular carcinomas lacking CDH1 genetic alterationsnpj Precision OncologyFebruary 12, 2024Higinio Dopeso, Andrea M. Gazzo, Fatemeh Derakhshan, David N. Brown, Pier Selenica, Sahar Jalali, Arnaud Da Cruz Paula, Antonio Marra, Edaise M. da Silva, Thais Basili, et al.labeled with Alexa-488 (E-Cadherin (#3199) Cell signaling, MA, USA; dilution 1:200). DAPI (Abcam # ab228549) was used as nuclear counterstaining. Fluorescent images were acquired using a TCS SP5 inverted confocal

(142) Decellularized Placental Sponge: A Platform for Coculture of Mesenchymal Stem Cells/Macrophages to Assess an M2 Phenotype and Osteogenic Differentiation In Vitro and In VivoACS OmegaFebruary 6, 2024Zahra Khosrowpour, Seyed Mahmoud Hashemi, Samira Mohammadi-Yeganeh, Sara Simorgh, Behnaz Sadat Eftekhari, Peiman Brouki Milan, Subhas C. Kundu, Mazaher Gholipourmalekabadiovernight. The samples were then incubated with a secondary antibody (ab6785, Abcam) for 1 h at RT. DAPI (ab228549, Abcam) was used to counterstain the cells’ nuclei. The cells were viewed under a fluorescence microscope

(143) The Fuzzy planar cell polarity protein (FUZ), necessary for primary cilium formation, is essential for pituitary developmentJournal of AnatomyFebruary 1, 2024Emily J. Lodge, William B. Barrell, Karen J. Liu, Cynthia L. Andoniadousheep serum for 1 h and then washed again. Finally, all slides were incubated in DAPI (1:5000, Abcam ab228549) in PBS for 10 min, then washed in PBS and coverslips were mounted with Vectashield Antifade Mounting

(144) The Lnc‐ENST00000602558/IGF1 axis as a predictor of response to treatment with tripterygium glycosides in rheumatoid arthritis patientsImmunity, Inflammation and DiseaseJanuary 16, 2024Yang Gao, Xiaoyue Wang, Yanfeng Gao, Jian Bai, Yanpeng Zhao, Renyi Wang, Hanzhou Wang, Guangzhao Zhu, Xixi Wang, Xiaochen Han, et al.conjugated fluorescent dye (Abcam, ab150129, 1:1000) at 37° for 1 h. After counterstaining with DAPI (Abcam, ab228549, 1:5000), images were captured with a Nikon Eclipse TS100 microscope equipped with Micro‐Manager 1.4.22

(145) Biotin-cGMP and -cAMP are able to permeate through the gap junctions of some amacrine cells in the mouse retina despite their large sizeFront Ophthalmol (Lausanne)January 15, 2024Chunxu Yuan, Luca Gerhards, Ilia A. Solov’yov, Karin DedekRRID: AB_2535792). To visualize cell nuclei, incubation in 4′,6-diamidino-2-phenylindole (DAPI, 1:1000, ab228549, Abcam) followed. After several washing steps, retinas were mounted on slides and coverslipped with

(146) Acute seizure activity in neonatal inflammation-sensitized hypoxia-ischemia in micePLOS ONEJanuary 11, 2024Angelina June, Weronika Matysik, Maria Marlicz, Emily Zucker, Pravin K. Wagley, Chia-Yi Kuan, Jennifer Burnsedon free-floating 40μm coronal sections [28]. 4’,6-diamidino-2-phenylindole (DAPI staining solution; ab228549; Abcam, Cambridge, United Kingdom) staining nuclear DNA was utilized to visualize anatomy and tissue

(147) The effect of human PBMCs immobilization on their Аβ42 aggregates-dependent proinflammatory state on a cellular model of Alzheimer''s diseaseFrontiers in neuroscienceJanuary 1, 2024Kot K, Kot Y, Kurbanov R, Andriiash H, Tigunova O, Blume Y, Shulga S.cell morphology was assessed by confocal microscopy after staining nuclear DNA with DAPI dye (Abcam, ab228549, λEx – 405 nm, λEm = 461 nm). The hPBMCs studied included all mononuclear blood cells, such as lymphocytes,

(148) CDK4/6 inhibitors display a class effect in inducing differentiation of neuroblastoma cellsWellcome open researchJanuary 1, 2024Ferguson KM, Abou Grealy FMY, Philpott A.blocking solution (1 hr, RT) and washed again. Nuclear counterstaining was performed using DAPI (Abcam, ab228549) (1:10000 in PBST, 15 min, RT). Imaging was performed on a Leica DMI 6000B Matrix microscope with Leica

(149) Cellular and molecular characterization of peripheral glia in the lung and other organsPloS oneJanuary 1, 2024Hall S, Liu S, Liang I, Schulz S, Ezran C, Tan M, Kuo CS.antibodies (Table 2). Sections were incubated with 4′, 6-diamidino-2-phenylindole (DAPI) (1:10,000, ab228549; Abcam, USA) for 1hr, fixed for 1h in 4% paraformaldehyde, washed with PBS, and placed in Cubic 1 solution

(150) Systematic assessment of transcriptomic and metabolic reprogramming by blue light exposure coupled with agingPNAS NexusDecember 5, 2023Jia Huang, Fan Zhou, Huanchan Zhou, Xiaoqi Zheng, Zhengyi Huo, Meng Yang, Zihe Xu, Runzhou Liu, Luoluo Wang, Xiaoyun Wang(#A-11008, Invitrogen) for 1 h. Tissues were mounted in PBT buffer and stained with DAPI Staining Solution (#ab228549, Abcam). A FLUOVIEW FV3000 confocal laser scanning microscope (Olympus, Shinjuku, Japan) was used to

(151) Omentum-derived matrix enables the study of metastatic ovarian cancer and stromal cell functions in a physiologically relevant environmentMatrix Biology PlusDecember 1, 2023Lisa J. Neilson, Douglas Cartwright, Maija Risteli, Elina M. Jokinen, Lynn McGarry, Toni Sandvik, Konstantina Nikolatou, Kelly Hodge, Samuel Atkinson, Maria Vias, et al.at 1:500 in 1 % BSA/0.1 % saponin in PBS for 1 h RT. Following a wash in 1 % BSA in PBS, Dapi (Abcam ab228549) at 1:2000 in 1 % BSA in PBS was applied for 20 min at room temperature. After a final wash in 1 % BSA

(152) Single-cell profiling reveals transcriptomic signatures of vascular endothelial cells in non-healing diabetic foot ulcersFront Endocrinol (Lausanne)December 1, 2023Yangzhou Lu, Xiaogang Liu, Jingling Zhao, Fan Bie, Yiling Liu, Julin Xie, Peng Wang, Junyou Zhu, Yahui Xiong, Shitian Qin, et al.Biosciences,Servicebio,Servicebio,Abcam Catalog Number,AF6191,AF0931,DF6191,AF1009,AF5176,S0001,G1222,G1223,ab228549 Dilution,1:200,1:200,1:100,1:200,1:100,1:200,1:500,1:500,1:10000 Serum sPD-L1 changes before and

(153) Influence of metabolic stress and metformin on synaptic protein profile in SH‐SY5Y‐derived neuronsPhysiological ReportsNovember 27, 2023Alex J. T. Yang, Ahmad Mohammad, Michael S. Finch, Evangelia Tsiani, Gaynor Spencer, Aleksandar Necakov, Rebecca E. K. MacPhersonbuffer containing phalloidin actin stain (Abcam; ab176757 (Wang et al., 2022)) and DAPI stain (Abcam; ab228549). Images were captured using a Biotek Cytation5 cell imaging reader. Immunofluorescent (IF) images were

(154) INO80 function is required for mouse mammary gland development, but mutation alone may be insufficient for breast cancerFrontiers in Cell and Developmental BiologyNovember 1, 2023Nguyen Xuan Thang, Dong Wook Han, Chanhyeok Park, Hyeonji Lee, Hyeonwoo La, Seonho Yoo, Heeji Lee, Sang Jun Uhm, Hyuk Song, Jeong Tae Do, et al.Abcam)] in the dark for 1 h, washed with 1X PBS thrice, and briefly counter-stained with DAPI (1:1000, #ab228549; Abcam). The sections were mounted and imaged using a confocal microscope (LSM800; Carl Zeiss, Oberkochen,

(155) Follicle-stimulating hormone orchestrates glucose-stimulated insulin secretion of pancreatic isletsNature CommunicationsNovember 1, 2023Yi Cheng, Hong Zhu, Jun Ren, Hai-Yan Wu, Jia-En Yu, Lu-Yang Jin, Hai-Yan Pang, Hai-Tao Pan, Si-Si Luo, Jing Yan, et al.fluorescent-conjugated secondary antibodies (1:500; Invitrogen, A21241, A11034). Nuclei were stained with DAPI (Abcam, ab228549). All immunofluorescence images were acquired by laser scanning confocal microscope (Zeiss). RNA interference Small

(156) Effects of oxytocin on the hair growth ability of dermal papilla cellsScientific ReportsOctober 20, 2023Tatsuto Kageyama, Jieun Seo, Lei Yan, Junji Fukuda°C and lastly with rhodamine-phalloidin (ab235138, Abcam) and 4′,6-diamidino-2-phenylindole (DAPI; ab228549, Abcam) in PBS for 30 min. A confocal microscope (LSM 700; Carl Zeiss, Germany) was used for fluorescence imaging

(157) Radiotherapy-Induced Neurocognitive Impairment Is Driven by Heightened Apoptotic Priming in Early Life and Prevented by Blocking BAXCancer researchOctober 13, 2023Singh R, Yu S, Osman M, Inde Z, Fraser C, Cleveland AH, Almanzar N, Lim CB, Joshi GN, Spetz J, et al.incubation at 1:200 dilution (Thermo Fisher Scientific A-11008, RRID:AB_143165) and DAPI staining (Abcam ab228549) was performed the next day, with cover slipping using vector labs antifade mounting media (Vector Laboratories

(158) Small Molecule Pytren-4QMn Metal Complex Slows down Huntington’s Disease Progression in Male zQ175 Transgenic MiceInternational Journal of Molecular SciencesOctober 13, 2023Marián Merino, Sonia González, Mª Carmen Tronch, Ana Virginia Sánchez-Sánchez, Mª Paz Clares, Antonio García-España, Enrique García-España, José L. Mullorantibody Alexa Fluor® 488 (ab150113; Abcam, Cambridge, UK) diluted 1:500 in blocking solution and DAPI (ab228549; Abcam, Cambridge, UK). Finally, the samples were washed three times with PBS 1× and assembled with

(159) Palbociclib releases the latent differentiation capacity of neuroblastoma cellsDevelopmental CellOctober 9, 2023Kirsty M. Ferguson, Sarah L. Gillen, Lewis Chaytor, Evon Poon, Daniel Marcos, Roshna Lawrence Gomez, Laura M. Woods, Lidiya Mykhaylechko, Louis Elfari, Barbara Martins Martins da Costa, et al.BioReagents™,BP9706-100 Beta-mercaptoethanol,BDH,441433A Trypsin EDTA 0.25%,ThermoFisher Scientific,25200072 DAPI,Abcam,ab228549 DMSO,Santa Cruz Biotechnology,sc-358801 PowerUp™ SYBR™ Green Master Mix,ThermoFisher Scientific,A25742 Crystal

(160) Autophagy-amplifying nanoparticles evoke immunogenic cell death combined with anti-PD-1/PD-L1 for residual tumors immunotherapy after RFAJournal of nanobiotechnologyOctober 3, 2023Zhang S, Huang Y, Pi S, Chen H, Ye F, Wu C, Li L, Ye Q, Lin Y, Su Z.Goat anti-rabbit IgG H&L (Alexa Fluor® 488) (ab150077), Goat anti-rabbit IgG H&L (Alexa Fluor® 555) (ab150078), and DAPI Staining Solution (ab228549) from Abcam. The preparation of STF@ZIF-8/PEG-FA (SZP), BMS@ZIF-8/PEG-FA(BZP), and STF-BMS@ZIF-8/PEG-FA

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6/17/2020This DAPI Binds Strongly to Nuclei Samantha LanjewarIt stains nuclei well and is easy to use.

DAPI Staining Solution from Abcam

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DAPI Staining Solution from Abcam

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