DAPI Staining Solution from Abcam

Product Specs
ItemDAPI Staining Solution
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab228549
Quantity2 mL
Sample TypeAdherent cells, Suspension cells
TypeStain

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(181) Estradiol-17β [E2] stimulates wound healing in a 3D in vitro tissue-engineered vaginal wound modelJournal of Tissue EngineeringJanuary 27, 2023Sarah Shafaat, Sabiniano Roman Regueros, Christopher Chapple, Sheila MacNeil, Vanessa Hearndentissue sections were counterstained with DAPI (4′,6-diamidino-2-phenylindole, λex359 nm: λem457 nm) (ab228549) (1:800 dilution; Abcam) and mounted with DPX to be imaged with an epifluorescence microscope (Olympus

(182) Napyradiomycin A4 and Its Relate Compounds, a New Anti-PRV Agent and Their Antibacterial Activities, from Streptomyces kebangsaanensis WS-68302MoleculesJanuary 8, 2023Yani Zhang, Wei Fang, Kaimei Wang, Zhigang Zhang, Zhaoyuan Wu, Liqiao Shi, Fang Liu, Zhongyi Wan, Manli Liuantibody (ab3534), goat anti-rabbit IgG H&L (Alexa Fluor 488) (ab150077), and DAPI staining solution (ab228549) were purchased from Abcam. Cell Proliferation The supernatant was discarded upon Marc proliferation;

(183) Macrophages induce gingival destruction via Piezo1-mediated MMPs-degrading collagens in periodontitisFrontiers in immunologyJanuary 1, 2023Zhao T, Chu Z, Chu CH, Dong S, Li G, Wu J, Tang C.hour at room temperature under protection from light. Next, add 4’,6-diamidino-2-phenylindole (DAPI) (ab228549; Abcam, USA) to stain the nuclei and incubate for 10 minutes at room temperature in the dark. Finally,

(184) The mitochondrial Ca(2+) channel MCU is critical for tumor growth by supporting cell cycle progression and proliferationFrontiers in cell and developmental biologyJanuary 1, 2023Fernandez Garcia E, Paudel U, Noji MC, Bowman CE, Rustgi AK, Pitarresi JR, Wellen KE, Arany Z, Weissenrieder JS, Foskett JK.Scientific, R37174), according to manufacturer’s instructions. After 15-min incubation, 4 mM DAPI (Abcam, ab228549) was added to the suspension and cells were analyzed by FACS with emission filters 530 and 440 nm for

(185) Intestinal epithelial stem cell transplants as a novel therapy for cerebrovascular strokeBrain, behavior, and immunityJanuary 1, 2023Mani KK, El-Hakim Y, Branyan TE, Samiya N, Pandey S, Grimaldo MT, Habbal A, Wertz A, Sohrabji F.in PBS, and cover slipped with mounting media containing the nuclear dye DAPI (Fluoroshield, Abcam, ab228549). Sections were imaged on the FV12-IX83 confocal microscope. 2.16. Villin, Ki67 quantification Gut

(186) Characterizing nuclear morphology and expression of eNOS in vascular endothelial cells subjected to a continuous range of wall shear stress magnitudes and directionalityJournal of the Mechanical Behavior of Biomedical MaterialsJanuary 1, 2023Jaideep Sahni, Mehwish Arshad, Morgan A. Schake, Justin R. Brooks, Ruiguo Yang, Peter D. Weinberg, Ryan M. Pedrigi(ab150117, Abcam) at 1:700 for 1 h at room temperature. Cells were incubated with 10 μM DAPI nuclear stain (ab228549, Abcam) for 2 min. All antibodies were diluted in blocking buffer and incubation steps were conducted

(187) Lumican silencing ameliorates β-glycerophosphate-mediated vascular smooth muscle cell calcification by attenuating the inhibition of APOB on KIF2C activityOpen medicine (Warsaw, Poland)January 1, 2023Li H, Zhang C, Liu Q.Fluor® 488 (ab150077; Abcam) and Alexa Fluor® 647 (ab150115; Abcam). Nuclei were labeled with DAPI (ab228549; Abcam). Protein images were captured using Zeiss LSM 710 confocal microscope (Carl Zeiss AG, Oberkochen,

(188) Parkia speciosa Hassk. Empty Pod Extract Prevents Cardiomyocyte Hypertrophy by Inhibiting MAPK and Calcineurin-NFATC3 Signaling PathwaysLife (Basel, Switzerland)December 23, 2022Mustafa NH, Jalil J, Saleh MSM, Zainalabidin S, Asmadi AY, Kamisah Y.and rinsed with PBS. The cellular nuclei were stained blue with 4′,6-diamidino-2-phenylindole (DAPI; ab228549, Abcam, Cambridge, MA, USA) and analyzed under a fluorescence microscope (Olympus Optical, Tokyo, Japan). 2.11.

(189) Gabapentin Increases Intra-Epidermal and Peptidergic Nerve Fibers Density and Alleviates Allodynia and Thermal Hyperalgesia in a Mouse Model of Acute Taxol-Induced Peripheral NeuropathyBiomedicinesDecember 8, 2022Michal Klazas, Majdi Saleem Naamneh, Wenhua Zheng, Philip Lazarovicigene-related peptides-I/II (CGRP-I/II) antibody [EPR23804-95] (ab283568) and DAPI staining solution (ab228549) were purchased from Abcam, Cambridge, UK). Alexa Fluor 594 affinity pure donkey (red, 112-585-167)

(190) Chronic Intermittent Hypoxia during Sleep Causes Browning of Interscapular Adipose Tissue Accompanied by Local Insulin Resistance in MiceInternational Journal of Molecular SciencesDecember 7, 2022Tehila Dahan, Shahd Nassar, Olga Yajuk, Eliana Steinberg, Ofra Benny, Nathalie Abudi, Inbar Plaschkes, Hadar Benyamini, David Gozal, Rinat Abramovitch, et al.MO, USA), Alexa Fluor®647 phalloidin (#ab176759, Invitrogen, Waltham, MA, USA), and DAPI (1:10,000, #ab228549, Abcam, Cambridge, UK). 4.6. Western Blot Analysis Tissues were homogenized in lysis buffer with protease

(191) In vitro neurons learn and exhibit sentience when embodied in a simulated game-worldNeuronDecember 7, 2022Brett J. Kagan, Andy C. Kitchen, Nhi T. Tran, Forough Habibollahi, Moein Khajehnejad, Bradyn J. Parker, Anjali Bhat, Ben Rollo, Adeel Razi, Karl J. FristonUSA) were incubated for 2 h. This was followed by 10 min of DAPI Staining Solution in PBS (1:1000, ab228549, Abcam, Cambridge, MA, USA) after which point slides were cover-slipped with ProLong Gold Antifade Mountant

(192) Spatial Analysis of Neural Cell Proteomic Profiles Following Ischemic Stroke in Mice Using High-Plex Digital Spatial ProfilingMolecular NeurobiologyDecember 1, 2022Jessica M. Noll, Catherine J. Augello, Esra KÃ¼rÃ¼m, Liuliu Pan, Anna Pavenko, Andy Nam, Byron D. Fordfor 2 min. Sections were then incubated in a freshly prepared solution of 0.0004% FJB with DAPI (Cat#ab228549, Abcam, Waltham, MA) for 20 min, rinsed in distilled water 3 times for 2 min, and then dried at 50 °C. For

(193) A multi-axis robot-based bioprinting system supporting natural cell function preservation and cardiac tissue fabricationBioactive materialsDecember 1, 2022Zhang Z, Wu C, Dai C, Shi Q, Fang G, Xie D, Zhao X, Liu YJ, Wang CCL, Wang XJ.then incubated with the secondary antibodies for 1 h at RT, counterstained with 0.5 μM DAPI (Abcam #ab228549) for 15 min at RT, and washed with 1 × PBS for 3 times. Coverslips and slides were mounted using Prolong Gold Antifade Reagent

(194) Targeting SOX10-deficient cells to reduce the dormant-invasive phenotype state in melanomaNature CommunicationsDecember 1, 2022Claudia Capparelli et al.secondary antibody, Phalloidin-TRITC (Sigma, P1951, 1:500) to visualize the cytoplasm, and DAPI (Abcam, ab228549, 1:1000) to visualize nuclei. Coverslips were mounted and imaged using a 40X objective lens on a Nikon

(195) Aptamer engineering exosomes loaded on biomimetic periosteum to promote angiogenesis and bone regeneration by targeting injured nerves via JNK3 MAPK pathwayMaterials Today BioDecember 1, 2022Yanlin Su, Qing Gao, Rongli Deng, Lian Zeng, Jingyi Guo, Bing Ye, Jialin Yu, Xiaodong Guoantibodies (8889S, Cell Signaling Technology, Beverly, MA, USA) for 1 h. After staining with DAPI (ab228549, Abcam, USA) for 5 min and observed with a fluorescent microscope (IX73, Olympus, Tokyo, Japan). Quantitative

(196) Spatially resolved proteomics via tissue expansionNature CommunicationsNovember 30, 2022Lu Li, Cuiji Sun, Yaoting Sun, Zhen Dong, Runxin Wu, Xiaoting Sun, Hanbin Zhang, Wenhao Jiang, Yan Zhou, Xufeng Cen, et al.23 °C. The sections were then washed three times with PBS and counterstained with DAPI (5 μM in PBS; ab228549; Abcam, US), before being mounted with Prolong Gold antifade reagent (ThermoFisher; US). Tissue expansion

(197) Tunable and Compartmentalized Multimaterial Bioprinting for Complex Living Tissue ConstructsACS Applied Materials & InterfacesNovember 23, 2022Shabir Hassan, Eduardo Gomez-Reyes, Eduardo Enciso-Martinez, Kun Shi, Jorge Gonzalez Campos, Oscar Yael Perez Soria, Eder Luna-Cerón, Myung Chul Lee, Isaac Garcia-Reyes, Joshua Steakelum, et al.Millipore Sigma. Primary antibodies for VE-cadherin (ab33168), MyHC (ab1242015), F-actin (ab205), and DAPI (ab228549) were purchased from Abcam (Cambridge, U.K.) and used according to the manufacturer’s protocol. PECAM-1

(198) Salidroside attenuates HALI via IL-17A-mediated ferroptosis of alveolar epithelial cells by regulating Act1-TRAF6-p38 MAPK pathwayCell communication and signaling : CCSNovember 21, 2022Guo B, Zuo Z, Di X, Huang Y, Gong G, Xu B, Wang L, Zhang X, Liang Z, Hou Y, et al.antibody (E032620, 1:400, Earthox) for 2 h at room temperature. Counterstaining of nuclei with DAPI (ab228549, Abcam) was also performed. Then slides were observed, and the images were captured with a fluorescence microscope

(199) Synaptic proteomics reveal distinct molecular signatures of cognitive change and C9ORF72 repeat expansion in the human ALS cortexActa neuropathologica communicationsOctober 29, 2022Laszlo ZI, Hindley N, Sanchez Avila A, Kline RA, Eaton SL, Lamont DJ, Smith C, Spires-Jones TL, Wishart TM, Henstridge CM.1:50 donkey anti-rabbit Alexa fluor 594–ab150076, Abcam, respectively) for 30 min and DAPI (1:1000, ab228549, Abcam) for 5 min. Samples were then mounted onto slides with Immumount mounting media. Images were

(200) Underwater instant adhesion mechanism of self-assembled amphiphilic hemostatic granular hydrogel from Andrias davidianus skin secretioniScienceOctober 21, 2022Yuqing Liu, Yinghao Li, Haitao Shang, Wen Zhong, Quan Wang, Kibret Mequanint, Chuhong Zhu, Malcolm Xing, Hong WeiFluor 568-conjugated secondary antibodies,Abcam,"Abcam Cat# ab175471, RRID:AB_2576207" DAPI,Abcam,ab228549 "Sprague Dawley (SD) rats, 6-week-old",The Animal Experimental Center of Third Military Medical University,N/A ,, "Chemicals,

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6/17/2020This DAPI Binds Strongly to Nuclei Samantha LanjewarIt stains nuclei well and is easy to use.

DAPI Staining Solution from Abcam

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DAPI Staining Solution from Abcam

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