EdU Assay / EdU Staining Proliferation Kit (iFluor 647) ab222421 from Abcam

Product Specs
ItemEdU Assay / EdU Staining Proliferation Kit (iFluor 647)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab222421
Quantity50 Test
Sample TypeAdherent cells, Suspension cells
TypeStain

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(1) Long noncoding RNA SRRM2-AS1 mediates coronary artery damage in Kawasaki disease by promoting endothelial-to-mesenchymal transitionClinical and Experimental MedicineFebruary 24, 2026Shiyang Song, Hui Zhang, Renchao Lin, Jingyi Zhang, Qianqian Zhang, Changhao Li, Yushan Xia, Jingjing Zeng, Kongchang Lu, Donghui Ge, et al.cell proliferation To detect cell proliferation, a 5-ethynyl-2-deoxyuridine (EdU) Assay kit (Cat. No. ab222421, Abcam) was used. Logarithmic growth phase cells were transferred to 96-well plates (4 × 103 to 1 ×

(2) Lipidomic profiling of human bile distinguishes cholangiocarcinoma from benign bile duct diseases with high specificity and sensitivity: a prospective descriptive studyBritish Journal of CancerNovember 30, 2025Fu-Sheng Liu, Ying-Yi Liu, Shi-Kun Zhang, Jun-Yu Zhou, Jing-Hua Li, Xiao-mian Li, Ming-He Zhang, Xiao-Yu Pan, Yi-Bo Chai, Wei-Xian Fang, et al.μM. The assays were conducted at 24, 48, 72, and 96 hours. For the EdU cell proliferation analysis (ab222421, Abcam, UK), the cells were incubated with 10 μM EdU after stimulation with 100 μM lipids for 48 hours.

(3) Geometrically Controlled WNT Activation Drives Intestinal MorphogenesisAdvanced Healthcare MaterialsSeptember 1, 2025Nathaniel C. Burmas, Arlyn M. Fabian, Amoli Vanavadiya, Quinton SmithProliferation Assay EdU assay was performed according to the manufacturer's instructions using the kit (Abcam ab222421). Prior to performing the assay, samples were incubated for 3 h at 37 °C with the EdU additive provided.

(4) EP300 Modulates MCM8 Transcription and Augments the Malignant Phenotype of Hepatitis B Virus–Positive Hepatocellular Carcinoma CellsThe Kaohsiung Journal of Medical SciencesMarch 17, 2025Fang Xue, Tian‐Feng Sunmicroplate reader. 2.8 Analysis of HCC Cell Viability Following the protocol of the EdU Assay Kit (ab222421, Abcam), the cells were cultured in 24‐well plates (5 × 104 cells per well) for 24 h. After a 2‐h incubation

(5) Nuclear receptor subfamily 4 group a member 1 eases angiotensin II-arose oxidative stress in vascular smooth muscle cell by boosting nucleotide-binding oligomerization domain-like receptor family caspase recruitment domain containing 3 transcriptionCytojournalNovember 16, 2024Li Shen, Feng Li, Ke Xia, Lingli Zhan, Dan Zhang, Zhiqiang YanSwitzerland). 5-Ethynyl-2’-deoxyuridine Cell growth was determined using the 5-ethynyl-2’-deoxyuridine (EdU) kit (Cat. #ab222421, Abcam, Cambridge, UK). A total of 10 µM of EdU was added to cells at a confluence level of 60–70% for

(6) Circ_001653 alleviates sepsis associated-acute kidney injury by recruiting BUD13 to regulate KEAP1/NRF2/HO-1 signaling pathwayJournal of inflammation (London, England)September 17, 2024Li X, Zhou W, Chen J, Zhou L, Li Y, Wu X, Peng X.proliferation rate of HK-2 cells was determined using an EdU Staining Proliferation Kit (iFluor 647) (ab222421, Abcam, USA). Briefly, HK-2 cells were grown in 96-well plates and cultured for one day. Then cells

(7) 17β‐estradiol promotes the progression of temporomandibular joint osteoarthritis by regulating the FTO/IGF2BP1/m6A‐NLRC5 axisImmunity, Inflammation and DiseaseAugust 2, 2024Xintong Xue, Changyi Li, Shuang Chen, Yan Zheng, Fan Zhang, Yan XuVazyme. Antibody diluents (A1800 and A1820) were purchased from Solarbio. The EdU proliferation kit (ab222421) was purchased from Abcam. Rat IL‐1β ELISA kit (J22380) and IL‐6 ELISA kit (J22434) were obtained from

(8) Microenvironmental stiffness induces metabolic reprogramming in glioblastomaCell ReportsOctober 1, 2023Alireza Sohrabi, Austin E.Y.T. Lefebvre, Mollie J. Harrison, Michael C. Condro, Talia M. Sanazzaro, Gevick Safarians, Itay Solomon, Soniya Bastola, Shadi Kordbacheh, Nadia Toh, et al.commercial assays,Critical commercial assays,Critical commercial assays EdU proliferation kit,Abcam,CAT# ab222421 Deposited data,Deposited data,Deposited data RNA-sequencing data,This paper,GEO accession number GSE239610 Experimental models

(9) Connexin 37 sequestering of activated-ERK in the cytoplasm promotes p27-mediated endothelial cell cycle arrestLife science allianceAugust 1, 2023Acharya BR, Fang JS, Jeffery ED, Chavkin NW, Genet G, Vasavada H, Nelson EA, Sheynkman GM, Humphries MJ, Hirschi KK.Delta Ct method. For EdU proliferation assay, Abcam EdU Staining Proliferation Kit (iFlour 647; Cat# ab222421) was used, and the manufacturer’s protocol was followed. Cells expressing different Cx37 constructs

(10) Protocol Optimization for Direct Reprogramming of Primary Human Fibroblast into Induced Striatal NeuronsInternational journal of molecular sciencesApril 5, 2023Kraskovskaya N, Bolshakova A, Khotin M, Bezprozvanny I, Mikhailova N.10% formalin. The number of cells in S phase was detected with EdU proliferation kit (Abcam, Cat. No. ab222421). The cells’ staining was detected by flow cytometry (Cytoflex, Beckman Coulter, Brea, CA, USA), and

(11) Thrombospondin-2 holds prognostic value and is associated with metastasis and the mismatch repair process in gastric cancerBMC CancerDecember 1, 2022Xiao-dong Chu, Zheng-bin Lin, Ting Huang, Hui Ding, Yi-ran Zhang, Zhan Zhao, Shu-chen Huangfu, Sheng-hui Qiu, Yan-guan Guo, Xiao-li Chu, et al.transfected with si-1 or si-2 (100 nM, 48 h). An EdU staining proliferation kit was purchased from Abcam (ab222421). EdU solution was added to the plates. Plates were incubated for 3 h and then treated with 4% formaldehyde.

(12) Poly ADP-ribosylation of SET8 leads to aberrant H4K20 methylation in mammalian nuclear genomeCommunications biologyNovember 25, 2022Estève PO, Sen S, Vishnu US, Ruse C, Chin HG, Pradhan S.Zeiss). DNA replication sites were detected using EDU Staining Proliferation Kit (iFluor 647, ABCAM # ab222421) on GFP-SET8 full-length transfected COS-7 cells according to manufacturer’s recommendations. Before

(13) LIMS2 is Downregulated in Osteosarcoma and Inhibits Cell Growth and MigrationJournal of OncologyOctober 13, 2022Chenying Su, Xiaona Cai, Taotao Xu, Yungang Wu, Licong Wang, Pinjie Chen, Chenxian Superformed to assess cell proliferation using the EdU Assay/EdU Staining Proliferation Kit (cat no. ab222421, Abcam). Each well of the 24-well plate 6 × 104 cells were plated into each well of the 24-well plate

(14) Directed cell migration towards softer environmentsNature materialsSeptember 1, 2022Isomursu A, Park KY, Hou J, Cheng B, Mathieu M, Shamsan GA, Fuller B, Kasim J, Mahmoodi MM, Lu TJ, et al.they were prepared into fluorescence microscopy samples using an EdU Proliferation Assay Kit (Abcam, ab222421), according to the manufacturer’s instructions. Briefly, the cells were supplemented with 20 μM EdU

(15) METTL3 promotes non-small cell lung cancer (NSCLC) cell proliferation and colony formation in a m6A-YTHDF1 dependent wayBMC Pulmonary MedicineAugust 25, 2022Xuejun Dou, Zhiyuan Wang, Weiqiang Lu, Libin Miao, Yuefeng ZhaoGraphPad Prism 8.0. The EDU incorporation was performed using Edu staining proliferation kit (Abcam, ab222421). RNA immunoprecipitation (RIP) RIP was performed with EZ-Magna RIP RNA-Binding Protein Immunoprecipitation Kit

(16) Phenotypic and molecular states of IDH1 mutation-induced CD24-positive glioma stem-like cellsNeoplasia (New York, N.Y.)June 1, 2022Haddock S, Alban TJ, Turcan Ş, Husic H, Rosiek E, Ma X, Wang Y, Bale T, Desrichard A, Makarov V, et al.specific to the protein core of CD24 [41,42]. EdU proliferation assay An EdU proliferation kit (Abcam, ab222421) was used according to the manufacturer's instructions, with the following changes: 4% paraformaldehyde

(17) The PARP1 Inhibitor Niraparib Represses DNA Damage Repair and Synergizes with Temozolomide for Antimyeloma EffectsJournal of OncologyApril 5, 2022Hong-Yuan Shen, Hai-Long Tang, Yan-Hua Zheng, Juan Feng, Bao-Xia Dong, Xie-Qun Chenapoptotic or necrotic cells. 2.5. EdU Assay An EdU Staining Proliferation Kit (iFluor 647) (Abcam, ab222421) was utilized in these assays. After drug treatment, the culture medium was supplemented with 20 μM

(18) A Substance P (SP)/Neurokinin-1 Receptor Axis Promotes Perineural Invasion of Pancreatic Cancer and Is Affected by lncRNA LOC389641Journal of immunology researchJanuary 1, 2022Ji T, Ma K, Wu H, Cao T.and 48 h using a microplate reader (Biotech, China). An EdU Proliferation Assay Kit (iFluor 647) (#ab222421; Abcam, Cambridge, MA, UK) was used to detect the proliferation rates of BxPC-3 and MIAPaCa-2 cells.

(19) FOXA1 Leads to Aberrant Expression of SIX4 Affecting Cervical Cancer Cell Growth and ChemoresistanceAnalytical cellular pathology (Amsterdam)January 1, 2022Wang Z, Sun BS, Chen ZS, Zhao KK, Wang YL, Meng FX, Zhang Y.plate was counted. 2.6. 5-Ethynyl-2′-Deoxyuridine (EdU) Assay The EdU Staining Proliferation kit (ab222421, Abcam) was applied to assess the proliferation of HT-3/DDP and HeLa/DDP cells, and all operations were

(20) Pharmacological targeting of Sam68 functions in colorectal cancer stem cellsiScienceDecember 17, 2021Masibag AN, Bergin CJ, Haebe JR, Zouggar A, Shah MS, Sandouka T, Mendes da Silva A, Desrochers FM, Fournier-Morin A, Benoit YD.affinity pulldowns,ThermoFisher,Cat# 14307D EdU Staining Proliferation Kit (iFluor 647),Abcam,Cat# ab222421 CellEvent™ Caspase-3/7 Green Detection Reagent,Thermo Fisher,Cat# C10423 Chromatin IP DNA Purification

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EdU Assay / EdU Staining Proliferation Kit (iFluor 647) from Abcam

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EdU Assay / EdU Staining Proliferation Kit (iFluor 647) from Abcam

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