Fig 1: Histopathology and immunohistochemistry of NiV-infected AGM tissuesRepresentative immunohistochemistry (IHC) images for anti-NiV N antibody (red) in AGM challenged with NiV and euthanized on 3 DPI (A–C), 4 DPI (D–F, J, and K), 5 DPI (G–I and M–O). IHC images for dual staining of anti-CD209 antibody (brown) and anti-NiV N antibody (red) (B, E, and H). IHC image for dual staining of anti-CD68 antibody (brown) and anti-NiV N antibody (red) (A and C). IHC image for anti-fibrin antibody (red) (L). Viral antigen IHC positive epithelium and dual stain NiV N and CD68 positive (A, arrows) within the tonsil at 3 DPI (A [AGM3-3]). Viral antigen IHC positive epithelium (arrow) and mononuclear cells within the tonsillar parenchyma in 4 DPI (D [AGM4-3]), and 5 DPI (G [AGM5-2]). Individual dual staining tonsillar antigen-presenting cells with anti-CD209 antibody (brown) and anti-NiV N antibody (red) in 3 DPI (B [AGM3-3]), 4 DPI (E [AGM4-3]), and 5 DPI (H [AGM5-4]) AGMs. Viral antigen IHC positive alveolar septa and dual stain NiV N and CD68 positive (C, arrow) within the lung at 3 DPI (C [AGM3-2]). Viral antigen IHC positive alveolar septa of the lung in 4 DPI (F [AGM4-2, arrows, increased alveolar macrophages]), and 5 DPI (I [AGM5-1, arrows, medium caliber vessels]) AGMs. IHC positive for fibrin in medium caliber vessels (L, arrow) and within pulmonary alveolar spaces (L AGM5-4). Viral antigen IHC positive mononuclear cells of the mandibular lymph node and spleen, respectively, in 4 DPI (J [AGM4-4], K [AGM4-2, arrow syncytial cells]), and 5 DPI (M [AGM5-2], N [AGM5-1, arrow syncytial cells]) AGMs. NiV N antigen IHC positive in the endothelium of the kidney in 5 DPI (O [AGM5-1]) AGMs. Images captured at 10× (G), 20× (L, M, and N), 40× (A, D, I, K, and O), and 60× (B–C, E, F, H, and J) magnifications. Scale bars represent 100 μm for 10× and 20× images, and 10 μm for 40× and 60× images.
Fig 2: Transcriptomic analyses of circulating blood, tonsil, and lung AGM exposed to NiVBVolcano plot showing the eight most highly upregulated mRNAs in circulating blood at 3 DPI (A), as quantified by nanostring targeted transcriptomics. Total number of significantly differentially expressed genes in tonsil (B) and lung (E), binned by day postexposure. Key enriched functional and signaling pathways in tonsil (C) and lung (F). Expression profiles for selected marker genes for viral RNA (NiV), complement cascade (C5 and C9), dendritic cells (CD209/DC-SIGN), and fibrin (FGB and FGG) in tonsil (D) and lung (G). For panel (A), n = 12 animals. For (B–G), the 3 DPI cohort was n = 12, the 4 DPI cohort was n = 8, and the 5 DPI cohort was n = 4. See also Figure S2.
Supplier Page from BioLegend for LEGENDplex™ HU Fibrinolysis Panel (5-plex) w/ VbP