Description
Applicable to a variety of pharmaceutical antibodies, the nSMOL (nano-surface and molecular orientation limited proteolysis) Antibody Bio-Analysis Kit enables selective proteolysis of the Fab region of monoclonal antibodies to dramatically improve the productivity and robustness of LCMS mAB bioanalysis.
This ready-to-use reagent kit is for collecting monoclonal antibodies from blood or other biological samples using immunoglobulin collection resin, and then performing selective proteolysis of the Fab region of these antibodies via trypsin-immobilized nanoparticles. Variable region-derived peptides produced by limited proteolysis can then be quantified via MRM measurements utilizing Shimadzu’s high-performance LCMS-8050/8060 triple quadrupole liquid chromatograph mass spectrometer. The simplified workflow eliminates the steps of denaturing, reduction, and alkyation normally associated with protein digestion, resulting in more efficient sample preparation and analysis.
Key benefits include:
- Faster, less expensive method development – Dramatically improves response and quantitative repeatability; no capture antibodies or ligands required.
- nSMOL proteolysis – Offers selective collection of Fab peptides and limits contamination from excessive peptides or trypsin.
- Highly sensitive and accurate assays are possible for a variety of antibodies.
- Applicable to a wide variety of pharmaceutical antibodies.