Description
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Salbutamol antigen. Standards or samples are added to the appropriate microtiter plate wells with Salbutamol specific antibody and Horseradish Peroxidase (HRP) conjugated anti-antibody. The competitive inhibition reaction is launched between pre-coated Salbutamol in standards or samples with the Salbutamol special antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of Salbutamol in the standards or samples. The color development is stopped and the intensity of the color is measured.
Background/Introduction: Salbutamol is a short-acting beta2-adrenoreceptor agonist used as antiasthmatic. It can improve the animal of lean meat and reduce fat content by adding micro-Salbutamol into feed, with higher toxicity than Ractopamine, therefore, Salbutamol is banned from using. The method of instrumental analytical is major to detect Salbutamol, but it needs expensive instruments, professional operators and complex pre-treatment; While the method of enzyme linked immunoassay has advantages with simple, rapid, high sensitivity, good specificity and low cost. This kit is a detection product developed based on ELISA technology, with operation time as short as 50 min and a sensitivity of 0.1 ppb, and linear range from 0.1 ppb to 8.1 ppb