Description
Description: This assay employs a two-site sandwich ELISA to quantitate SEPT2 in samples. An antibody specific for SEPT2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SEPT2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SEPT2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SEPT2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: SEPT2 can hetero-oligomerize wth SEPT6 and SEPT7 to form filaments. SEPT2 interacted with SEPT6 through its C-terminal coiled-coil domain. Knockdown of SEPT2, SEPT6, and SEPT7 in causes actin stress fibers to disintegrate and cells to lose polarity. The NEDD5 cDNA encodes a predicted protein of 406 amino acids. The deduced peptide sequence contains conserved domains rich in basic residues, a motif of the GTPase superfamily. Different poly(A) sites account for generation of 2 transcripts. The major 3.5-kb transcript was expressed ubiquitously in all human tissues examined, and a 2.0-kb alternative transcript lacking any long AU-rich element in the 3-prime noncoding region was expressed abundantly only in testis, heart, and skeletal muscle