Description
Description: This assay employs a two-site sandwich ELISA to quantitate F12 in samples. An antibody specific for F12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any F12 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for F12 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of F12 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: The main role of factor VII (FVII) is to initiate the process of coagulation in conjunction with tissue factor (TF). Tissue factor is found on the outside of blood vessels - normally not exposed to the bloodstream. Upon vessel injury, tissue factor is exposed to the blood and circulating factor VII. Once bound to TF, FVII is activated to FVIIa by different proteases, among which are thrombin (factor IIa), factor Xa, IXa, XIIa, and the FVIIa-TF complex itself.The most important substrates for FVIIa-TF are Factor X and Factor IX. The action of the factor is impeded by tissue factor pathway inhibitor (TFPI), which is released almost immediately after initiation of coagulation. Factor VII is vitamin K dependent; it is produced in the liver. Use of warfarin or similar anticoagulants impairs its function