Description
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Ochratoxin A antigen. Standards or samples are added to the appropriate microtiter plate wells with Ochratoxin A specific antibody and Horseradish Peroxidase (HRP) conjugated anti-antibody. The competitive inhibition reaction is launched between pre-coated Ochratoxin A and Ochratoxin A in standards or samples with the Ochratoxin A special antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of Ochratoxin A in the standards or samples. The color development is stopped and the intensity of the color is measured.
Background/Introduction: Ochratoxin A (OTA) is a toxic metabolite produced by Aspergillus flavus and Penicillium genera. Ochratoxin A has a strong liver and kidney toxicity, and has teratogenicity, mutagenicity and carcinogenicity. Ochratoxin A was considered to be the second important and harmful mycotoxin in the family following with Aflatoxin flavus. As the Ochratoxin A producing fungi exist widely in nature, especially higher yield in cereals and cereals products, coffee, cocoa contaminated with Ochratoxin A, it has been potential threat for human health and development of animal husbandry. This kit is a detection product developed based on ELISA technology, with operation time as short as 50 min and a sensitivity of 0.5 ppb, and linear range from 0.5 ppb to 40.5 ppb