Description
Principle of the Assay: beta-Hydroxybutyrate Assay Kit is a convenient quantitative tool that measures beta-Hydroxybutyrate within biological samples. The assay is based on an enzymatic cycling reaction in which the cofactor NAD+ is reduced to NADH. NADH reacts with a fluorometric probe that produces a product which can be measured with a standard 96-well fluorometric plate reader. The intensity of the product fluorescence is proportional to the beta-Hydroxybutyrate within a sample. Samples and standards are incubated for 30 minutes and then read at lambdaex = 530-570 nm/ lambdaem = 590-600 nm (Figure 1). Samples are compared to a known concentration of beta-Hydroxybutyrate standards within the 96-well microtiter plate format