Description
Description: This assay employs a two-site sandwich ELISA to quantitate OPN5 in samples. An antibody specific for OPN5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any OPN5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for OPN5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of OPN5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: Opsins are members of the guanine nucleotide-binding protein (G protein)-coupled receptor superfamily. This opsin gene is expressed in the eye, brain, testes, and spinal cord. OPN5 belongs to the seven-exon subfamily of mammalian opsin genes that includes peropsin (RRH) and retinal G protein coupled receptor (RGR). Like these other seven-exon opsin genes, this gene may encode a protein with photoisomerase activity. Alternative splicing results in multiple transcript variants encoding different isoforms.The deduced 472-amino acid protein contains the characteristic 7 transmembrane (TM) domains, a DRY motif in the intracellular side of TM3, and a rhodopsin GPR-like NPxxY motif in TM7. GPR136 shares 96% amino acid identity with mouse Gpr136. EST database analysis indicated that GPR136 is expressed in testis