Description
Description: This assay employs a two-site sandwich ELISA to quantitate YBX2 in samples. An antibody specific for YBX2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any YBX2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for YBX2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of YBX2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: YBX2 is a germ-cell specific member of the vertebrate Y box family of nucleic acid-binding proteins. The deduced 364-amino acid protein has a calculated molecular mass of 38.6 kD and is arginine- and proline-rich. It shares 69% and 73% amino acid identity with DBPA (CSDA) and YB1 (NSEP1), respectively, and contains an N-terminal cold-shock domain (CSD), 7 putative phosphorylation sites for casein kinase II, 3 potential phosphorylation sites for protein kinase C, and 2 nuclear localization signals within a B/A island. Northern blot analysis detected a 1.8-kb transcript in human testes only. YBX2 shares 93% and 77% amino acid identity with mouse Ybx2 and Xenopus Frgy2, respectively.The deduced 360-amino acid mouse Msy2 and 282-amino acid Msy2a isoforms have calculated molecular masses of 38 and 31 kD, respectively