Description
Description: This assay employs a two-site sandwich ELISA to quantitate GIPC3 in samples. An antibody specific for GIPC3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GIPC3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GIPC3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GIPC3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: By genomic sequence analysis and RT-PCR of fetal lung RNA, Saitoh et al. (2002) obtained a full-length cDNA encoding GIPC3. The predicted 312-amino acid protein contains a central PDZ domain. GIPC3 shares 59.9% amino acid identity with GIPC1, 55.3% identity with GIPC2, and 57.2% identity with Xenopus Kermit. Northern blot analysis detected nearly ubiquitous expression of a 4.5-kb GIPC3 transcript, with highest expression in small intestine. RNA dot blot analysis showed highest GIPC3 expression in jejunum, lymph node, parietal lobe, fetal spleen, and fetal thymus. Northern blot analysis detected GIPC3 expression in cervical, melanoma, and chronic myelogenous leukemia cancer cell lines and in 2 of 7 gastric cancer cell lines examined