Description
Description: This assay employs a two-site sandwich ELISA to quantitate FAM98A in samples. An antibody specific for FAM98A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FAM98A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FAM98A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FAM98A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: FAM98A, is a gene that in the human genome encodes the FAM98A protein. FAM98A has two paralogs in humans, FAM98B and FAM98C. All three are characterized by DUF2465, a conserved domain shown to bind to RNA. FAM98A is also characterized by a glycine-rich C-terminal domain. FAM98A also has homologs in vertebrates and invertebrates and has distant homologs in choanoflagellates and green algae. FAM98A is 518 amino acids in length with a molecular weight of 55.3 kDa, without modifications. Residues 10-329 comprise the DUF2465, and the remainder of the protein is a diglycine-rich C terminus. Glycine makes up approximately 20% of the protein, with the majority of these in the last 200 residues. FAM98A has six strongly predicted phosphorylation sites in DUF2465