Description
Principle of the Assay: The Mouse TNFRSF19/TROY ELISA Kit was based on standard sandwich enzyme-linked immunesorbent assay technology. A monoclonal antibody from rat specific for TNFRSF19/TROY has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: E30-L170) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFRSF19/TROY is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse TNFRSF19/TROY amount of sample captured in plate.
Background: Tumor necrosis factor receptor superfamily, member 19, also known as TNFRSF19 and TROY, is a human gene. It is mapped to 13q12.11-q12.3. The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is highly expressed during embryonic development. It has been shown to interact with TRAF family members, and to activate JNK signaling pathway when overexpressed in cells. This receptor is capable of inducing apoptosis by a caspase-independent mechanism, and it is thought to play an essential role in embryonic development. Alternatively spliced transcript variants encoding distinct isoforms have been described